A method for comprehensive evaluation of fecundity of male mice

A comprehensive evaluation and fecundity technology, applied in data processing applications, instruments, calculations, etc., can solve problems such as identification errors, and achieve the effects of simple operation, improved production efficiency, and reduced feeding costs

Active Publication Date: 2021-09-24
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these indicators are affected by the reproductive status of female mice, resulting in errors in identification

Method used

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  • A method for comprehensive evaluation of fecundity of male mice
  • A method for comprehensive evaluation of fecundity of male mice
  • A method for comprehensive evaluation of fecundity of male mice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] This embodiment is an example of the method for comprehensive evaluation of the fertility of male rats described in the present invention. In this embodiment, the C57BL / 6J male mouse is used as the identification object, and the experiment is repeated 3 times, and 2 male mice of different parents and different batches are used for each repetition, which can illustrate that the present invention does not require surgery, does not need to injure the reproductive organs of male mice, and is effective. Protect high-quality fecundity male rats, and can effectively and objectively identify high-quality male rats. Before female mice and male mice are caged for natural mating, it is necessary to record the estrous state of female mice at 4-5 weeks of growth and development, discard prematurely sexually mature female mice, and test vaginal smears to ensure that female mice are in natural estrus.

[0055] Including the following steps and specific implementation process:

[0056...

Embodiment 2

[0067] This embodiment is a specific embodiment of the evaluation criteria of the method for comprehensive evaluation of the fertility of male rats according to the present invention. This example is the actual operation of detecting the fecundity of C57BL / 6J male mice No. 2, No. 9, No. 10, No. 11, No. 13 and No. 17. The evaluation criteria are included in the data with scores, which are 0 points, 1 point, 2 points and 3 points. 0 points indicate that the observed phenomenon does not match the described phenomenon or the index value is very low; 1 point indicates that the observed phenomenon is similar to the described phenomenon but not obvious or The index value is low; 2 points indicate that the observed phenomenon is consistent with the described phenomenon or the index value is high; 3 points indicate that the observed phenomenon is consistent with the described phenomenon and is particularly obvious or the index value is very high. Make the judging standard table and the...

Embodiment 3

[0091] This embodiment is an example of the fertility effect of the method for comprehensive evaluation of the fertility of male rats and the judging criteria of the present invention. Screen the fecundity of No. 2, No. 9, No. 10, No. 11, No. 13 and No. 17 C57BL / 6J male mice, the mating data of male and female mice, statistics of vaginal status, gestational days, litter size and fetal survival number, explain Positive effect of identification of screened male fertility on production and selection of mice.

[0092] Table 2 Statistical table of judging data of each male mouse

[0093]

[0094]

[0095] It can be seen from the statistical table of the evaluation data of each male mouse in Table 2: record the vaginal status on the second day after mating, as well as the number of days of pregnancy, litter size and fetal survival number; compare the fertility evaluation of male mice in Table 1 according to these four indicators The short list of standards can be judged by sc...

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Abstract

A comprehensive evaluation system for the fertility of male mice and its evaluation criteria. Male mice and female mice that are 8-9 weeks old, healthy in appearance, sensitive in response, up to standard in weight, bright in coat color, and well-developed sexual organs are selected respectively. They are reared in single cages and fed ad libitum Breeding feed and drinking water, keeping light for 10 hours and dark for 14 hours to feed at intervals, discarding precocious puberty or female mice with vaginal ectropion opening at 4-5 weeks, vaginal smears to select estrous female mice, and naturally estrous female mice and selected male mice Rats were mated in a cage at a ratio of 1:1. After 10-12 hours in the cage, the vaginal plugs of the female mice were checked, placed in a quiet environment for careful care, and the number of gestational days, number of litters and surviving fetuses of the female mice were counted. The invention can effectively and accurately identify the fecundity of male rats, screen high-quality fecundity male rats, and effectively protect high-quality fecundity male rats from surgery; The standard table makes the test more analyzable and objective, and can visually judge the fertility of male mice.

Description

technical field [0001] The invention relates to the technical field of male rat fecundity identification and male rat breeding, in particular to a comprehensive evaluation method for male fecundity. Background technique [0002] The fertility of male mice has a profound impact on the offspring. The quality of male mice directly affects the production and research of mice, so the identification of male fertility plays a crucial role. In production and scientific research, the fecundity of male rats is ignored, and the fecundity of selected male rats is uneven, resulting in a low level of production efficiency, which will also greatly affect the results of scientific research and analysis and judgment. Whether the evaluation methods and criteria of male rat fecundity can accurately, effectively and objectively judge male rat fecundity plays a vital role. [0003] The reproductive system of male mice includes testes, epididymis, vas deferens, prostate and seminal vesicles. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02G06Q10/06
CPCA01K67/02G06Q10/0639
Inventor 孙俊铭欧阳轶强张名媛郭晓萍莫忠香梁宁李家富胡冰刘佳娟何萍
Owner GUANGXI MEDICAL UNIVERSITY
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