Online-internal-extraction electrospray-ionization mass-spectroscopy detection device for rapidly analyzing body fluid samples of human body

Abstract

The invention relates to an online-internal-extraction electrospray-ionization mass-spectroscopy detection device for rapidly analyzing body fluid samples of a human body, and belongs to the technical field of chemical analysis. The detection device consists of an eluate introduction device, an in-situ sample enriching extractor, a sample introduction device, an electrospray ionization source and a mass spectrograph, wherein the eluate introduction device is used for introducing a flowing phase for sample eluting, the in-situ sample enriching extractor is used for carrying out sample loading and online extracting and enriching, the sample introduction device is used for introducing eluted samples into the electrospray ionization source for electrospraying, and the mass spectrograph is used for carrying out detection and analysis on online samples, so as to obtain mass-spectroscopy molecular fingerprint spectrogram information on the samples. According to the detection device, the detected human body fluid samples may be human body fluids such as blood, urine, sweat and saliva, do not need sample pretreatment, and are subjected to in-situ sample loading. The device provided by the invention is simple in structure and low-cost, the practicability is high, the detection time and economic cost are reduced greatly, and a technical support is provided for the rapid diagnosis and screening of critical illnesses.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis, and in particular relates to an online internal extraction electrospray ionization mass spectrometry detection device for rapidly analyzing human body fluid samples. Background technique [0002] At present, in the face of a large number of complex matrix biological samples, the traditional sample detection process is complex and time-consuming, and the sample pretreatment process is time-consuming, which may easily cause sample loss, which is not conducive to the online monitoring of samples, and seriously restricts the rapid screening of samples. Especially in the early detection, early diagnosis and early treatment of major human diseases (such as tumors and cancers), rapid sample screening is of great significance for the diagnosis and treatment of diseases. At present, body fluid (such as blood or urine) is an important sample in disease diagnosis. The rapid development of "biopsy"...

AI Technical Summary

Problems solved by technology

[0002] At present, in the face of a large number of complex matrix biological samples, the traditional sample detection process is complicated and time-consuming, and the sample pretreatment process is time-consuming, which is easy to cause sample loss, which is not conducive to the online monitoring of samples, and seriously restricts the rapid screening of samples.

Images