EV71 virus IgA antibody detection test strip and application thereof

Abstract

The invention relates to an EV71 virus IgA antibody detection test strip which comprises a sample pad, a label pad, a nitrocellulose membrane, absorbing paper and a back plate. The nitrocellulose membrane is provided with an EV71 virus VP1 protein antigen peptide fragment detection line and a human IgA antibody control line. The label pad is provided with an an-human IgA anti-body-colloidal gold marker. The EV71 virus IgA antibody detection test strip is used for saliva EV71 virus detection; by detecting an IgA anti-body in a saliva sample, EV71 viruses can be quickly screened, reliability of detection results can be improved, and false-negative conditions are reduced. A detection method is simple to operate, the results are quick, the collection of a detected sample has no invasiveness, and discomfort and cross infection caused by conventional blood sampling and collection of throat swab, anus swab samples and cerebrospinal fluid are avoided.

Description

technical field [0001] The invention relates to a virus detection test strip and its application, in particular to a EV71 virus IgA antibody detection test strip and its application. Background technique [0002] Enterovirus 71 (referred to as EV71 virus) belongs to the family Picornaviridae Enterovirus, a non-enveloped single-stranded positive-sense RNA virus, and is one of the main pathogens causing hand-foot-mouth disease (HFMD). one. The EV71 virus can be transmitted through air droplets and other channels, and is highly pathogenic. It often causes severe central nervous system complications such as aseptic meningitis, encephalitis, poliomyelitis-like paralysis, and neurogenic pulmonary edema. Cause death, currently there is no effective treatment, mainly through early diagnosis and symptomatic treatment. EV71 virus mainly infects children under the age of 6, especially children aged 2-5. Most children in this age group attend kindergartens. Therefore, kindergartens ar...

AI Technical Summary

Problems solved by technology

[0004] Cell isolation and culture is the gold standard method for determining EV71 virus infection, but its clinical diagnostic application is limited by the shortcomings of long detection time (more than 5 days), high requirements for experimental conditions, and high technical requirements for operators.

[0005] RT-PCR nucleic acid molecular detection is the most sensitive method among the early detection methods of EV71 virus. To judge the infection status by detecting EV71 virus in throat swabs and anal swabs of patients, professional technicians and expensive fluorescent PCR amplification instruments are required. It is difficult for primary medical departments to carry out this diagnostic method

In addition, when collecting cells from the patient's throat, it is more likely to cause the patient's discomfort, regurgitation or vomiting, which makes the work of medical staff more difficult, and also easily increases the risk of virus contamination in the sampling room

Finally, the circulation process of the virus in the patient's body after EV71 virus infection is relatively complicated. According to the development of the disease, sometimes the virus can be detected in the throat cells to obtain results, and sometimes in the intestinal tract, the results can be obtained by detecting anal swabs. Sometimes it is in the throat and intestines at the same time, but sometimes the virus is neither in the throat nor in the intestines, so the RT-PCR results often have false negative results

[0006] Serological IgM antibody ELISA detection is mainly to understand the recent infection of patients by detecting EV71 virus IgM antibody in serum, which is helpful for clinical diagnosis and treatment. However, many EV71 virus patients are children under 6 years old, and the blood vessels of many young children It is thin and has a sense of fear for blood drawing, so it brings certain difficulties to the operation of clinical medical staff.

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