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A kind of collembola implantation culture solution and preparation method thereof

A culture medium and implantation technology, which is applied in the field of Collembola implantation culture medium and its preparation, can solve the problems of culture collapse, difficult control of water retention and adjustment of bacteria, and easy death, so as to stabilize the culture system and improve the ability to resist environmental interference , the effect of improving the success rate of domestication

Inactive Publication Date: 2020-03-17
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The collembola implantation culture solution provided by the present invention solves the problem of easy death when the wild species are domesticated and the breeding species is expanded, and it is difficult to control water retention and bacteria regulation during the artificial culture of collembola. The problem that the culture of collembola such as Baifutiao is prone to collapse due to bacterial outbreaks

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A collembola implantation culture solution, each liter of collembola implantation culture solution contains: 380ml soil Chlamydomonas cell number greater than 8×10 6 cells.ml -1 Soil Chlamydomonas solution, 0.47μg vitamin B12, 25.2mg vitamin C, 67.2mg dipotassium hydrogen phosphate, 189.5mg calcium hydrogen phosphate, 69.3mg magnesium sulfate heptahydrate, 137.6mg sodium glutamate, 14.7 mg of L-lysine, 95 mg of sucrose, and the solvent is cold boiled water, wherein the cold boiled water is obtained by boiling distilled water for more than 5 minutes and then cooling to room temperature.

[0030] Specifically follow the steps below:

[0031] Step 1, culture soil preparation: select the forest soil with Chlamydomonas provenance, remove the litter on the soil surface, take the surface humus 2cm below the ground, and remove the roots, stones and other sundries in the surface humus , get culture soil;

[0032]Step 2, soil Chlamydomonas provenance bag preparation: Weigh 40 ...

Embodiment 2

[0043] A collembola implantation culture solution, each liter of collembola implantation culture solution contains: 400ml soil Chlamydomonas cell number greater than 8×10 6 cells.ml -1 Soil Chlamydomonas solution, 0.5 μg of vitamin B12, 24 mg of vitamin C, 64 mg of dipotassium hydrogen phosphate, 210 mg of calcium hydrogen phosphate, 73.0 mg of magnesium sulfate heptahydrate, 131 mg of sodium glutamate, 14 mg of L-lysine Amino acid, 100 mg of sucrose, and the solvent is cold boiled water, wherein the cold boiled water is obtained by boiling distilled water for more than 5 minutes and then cooling to room temperature.

[0044] Specifically follow the steps below:

[0045] Step 1, culture soil preparation: select the forest soil with Chlamydomonas provenance, remove the litter on the soil surface, take the surface humus 1cm below the ground, and remove the roots, stones and other sundries in the surface humus , get culture soil;

[0046] Step 2, soil Chlamydomonas provenance ...

Embodiment 3

[0056] A collembola implantation culture solution, each liter of collembola implantation culture solution contains: 420ml soil Chlamydomonas cell number greater than 8×10 6 cells.ml -1 Soil Chlamydomonas solution, 0.53 μg of vitamin B12, 23.8 mg of vitamin C, 60.8 mg of dipotassium hydrogen phosphate, 220.5 mg of calcium hydrogen phosphate, 76.7 mg of magnesium sulfate heptahydrate, 124.4 mg of sodium glutamate, 13.3 mg of L-lysine, 105 mg of sucrose, and the solvent is cold boiled water, wherein the cold boiled water is obtained by boiling distilled water for more than 5 minutes and then cooling to room temperature.

[0057] Specifically follow the steps below:

[0058] Step 1, culture soil preparation: select the forest soil with Chlamydomonas provenance, remove the litter on the soil surface, take the surface humus 3cm below the ground, and remove the roots, stones and other sundries in the surface humus , get culture soil;

[0059] Step 2, soil Chlamydomonas provenance ...

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Abstract

The invention discloses a springtail implantation culture solution. Each liter of the springtail implantation culture solution contains 380-420ml of a soil chlamydomonas reinhardtii solution, 0.47-0.53[mu]g of vitamin B12, 23.8-25.2mg of vitamin C, 60.8-67.2mg of dipotassium hydrogen phosphate, 189.5-220.5mg of dicalcium phosphate, 69.3-76.7mg of magnesium sulfate heptahydrate, 124.4-137.6mg of sodium glutamate, 13.3-14.7mg of L-lysine and 95-105mg of cane sugar, and boiled water is used as a solvent. In addition, the invention further provides a preparation method of the springtail implantation culture solution. The springtail implantation culture solution disclosed by the invention can improve the environmental disturbance resistant capacity of a culture system and stabilize the culture system, so that the domesticating success rate of wild species can be notably increased; and in addition, the springtail implantation culture solution can replace conventional feeds such as yeast, and the cost can be notably reduced.

Description

technical field [0001] The invention belongs to the technical field of insect feed, and in particular relates to a collembola implantation culture solution and a preparation method thereof. Background technique [0002] At present, with the in-depth development of resource development and utilization, the demand for micro-feeds is increasing. Collembola, as a new type of micro-feed, is an important high-protein food for lizards, praying mantises, scorpions, centipedes, spiders, frogs, geckos, etc. However, due to the lack of technological breakthroughs in the domestication of wild springtails, only one species of Baifutiao has been successfully raised artificially among the more than 12,000 springtails reported worldwide. [0003] When Baifutiao is cultivated in the laboratory, gypsum-charcoal or sterilized soil is generally used as the water-retaining substrate, and high-protein feed such as yeast is used as the main food, and the addition of water and food is required duri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/90A23K10/30A23K20/174A23K20/26A23K20/142A23K20/163
Inventor 颜绍馗黄苛朱睦楠
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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