System and method for rapid three-dimensional refractive index microscopy imaging
A microscopic imaging and refractive index technology, applied in microscopes, instruments, optics, etc., can solve the problems of complex devices, high equipment requirements, difficult to recover three-dimensional refractive index information, etc., and achieve the effect of rapid acquisition
Active Publication Date: 2019-08-09
TSINGHUA UNIV
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Problems solved by technology
However, these methods have higher requirements on equipment and more complex devices, and each time the imaging process needs to take multiple shots of the sample, it is difficult to recover the three-dimensional refractive index information of the sample with a single shot. Its fast motion capture presents a challenge
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[0027] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary only for explaining the present invention and should not be construed as limiting the present invention.
[0028] In describing the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", " The orientations or positional relationships indicated by "vertical", "horizontal", "top", "bottom", "inner" and "outer" are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and Simplified descriptions, rather than indicating or implying that the device or element refe...
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The invention discloses a rapid three-dimensional refractive index micro-imaging system and method. The system comprises an illumination generation module, a microscopic sample, a micro-imaging module and a calculation control module, the illumination generation module is used for generating microscopic illumination with a light intensity code, and the light intensity code is a provided reference picture with texture; the microscopic sample is located at the back end of the illumination generation module, the micro-imaging module is located at the back end of the microscopic sample and used for conducting imaging acquisition on an emergent ray from multiple angles, the calculation control module is used for providing reference pictures, controlling an acquisition process, processing the acquired pictures, and restoring and reconstructing three-dimensional refractive information of the microscopic sample. The rapid three-dimensional refractive index micro-imaging system and method have the advantage that undistorted reference pictures not containing the sample and distorted reference pictures containing the sample are shot at all angles, and therefore the three-dimensional refractive index information of the microscopic sample is collected in high resolution precisely and rapidly.
Description
technical field [0001] The invention relates to the technical fields of microscopic imaging technology, computer vision and computational photography, in particular to a system and method for rapid three-dimensional refractive index microscopic imaging. Background technique [0002] The three-dimensional refractive index distribution information of the sample object is an important inherent optical property of the sample. For transparent biological samples, it can provide information such as the density and structure of the sample object, thus providing the possibility for label-free three-dimensional cell imaging. [0003] In existing life science or medical research, fluorescence imaging is often used for specific labeling to conduct related research, but this labeling process will still have a certain impact on the properties of the sample itself, thus affecting the final results. Experimental results. The three-dimensional refractive index imaging can non-invasively obs...
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IPC IPC(8): G02B21/00G02B21/06G02B21/36
CPCG02B21/0004G02B21/06G02B21/361G02B21/365
Inventor 索津莉张明捷吴嘉敏戴琼海
Owner TSINGHUA UNIV