Phase microscopy imaging system and method based on optical flow

Abstract

A phase microscopy system and method are provided in the present disclosure. The system includes: an optical source, configured to generate collimated light; an object arrangement component, configured to provide a mask and a sample; a microscopic imaging component, including a microscope and a sensor, the microscope being configured to project light that passes successively through the mask and the sample into the sensor to capture an image; and a control component, configured to reconstruct phase information of the sample based on the image through both the sample and the mash and a pre-stored reference image of the mask, in which the pre-stored reference image is pre-acquired by the phase microscopy system without providing the sample under a same light condition. With the present disclosure, dynamic phase information of the microscopic sample can be acquired accurately and rapidly with high resolution.

Description

technical field [0001] The invention relates to the technical fields of microscopic imaging technology, computer vision and computational photography, in particular to an optical flow-based phase microscopic imaging system and a method thereof. Background technique [0002] The phase of an object is an important piece of information in the optical field. It can provide information such as the shape and density of the object. When light passes through a colorless and transparent object, it does not cause a change in intensity, but often brings a The change. Therefore, phase imaging technology can restore and reconstruct the structure or refractive index of transparent objects through the collection of phase information, so that colorless and transparent sample objects can be clearly seen. It has a wide range of applications and has become a research hotspot in recent years. [0003] In recent years, various phase microscopy techniques have been proposed one after another, wh...

AI Technical Summary

Problems solved by technology

Among them, the interferometric phase microscopy imaging technology generally requires a highly coherent light source, that is, a relatively complex interferometric device, which limits the spatial resolution and measurement accuracy of the imaging system.

[0004] Incoherent phase microscopy technology does not use interference, but restores phase information based on the intensity information of multiple acquired images. The commonly used method is the light intensity transmission equation method. This method can avoid the use of complex interference devices, but It often needs to take multiple different defocused images, which poses a challenge to its dynamic shooting, and the method of single image restoration often sacrifices the spatial resolution of the imaging system

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