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IP10 single-chain antibody targeting tumor vascular endothelial cells and preparation method thereof

A single-chain antibody and tumor blood vessel technology, applied in the medical field, can solve the problem of not being able to enrich IP10, achieve the effects of inhibiting tumor growth, promoting angiogenesis, and prolonging survival time

Inactive Publication Date: 2020-08-18
GUANGXI MEDICAL UNIVERSITY
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide an IP10 single-chain antibody targeting tumor vascular endothelial cells and its preparation method and use, which can not only specifically target To bind tumor vascular endothelial cells, and can activate lymphocytes in the body or adopt CIK cells to tumor vascular endothelial cells, and participate in the annihilation of tumor cells

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  • IP10 single-chain antibody targeting tumor vascular endothelial cells and preparation method thereof
  • IP10 single-chain antibody targeting tumor vascular endothelial cells and preparation method thereof
  • IP10 single-chain antibody targeting tumor vascular endothelial cells and preparation method thereof

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Embodiment Construction

[0037] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0038] Endoglin, also known as CD105, is a cell membrane glycoprotein that is mainly distributed on the surface of endothelial cells of new blood vessels and vascular endothelial cells of surrounding tissues. Therefore, Endoglin is closely related to tumor angiogenesis and is one of the hallmark molecules of tumor angiogenesis. Endoglin, as a target for tumor therapy, is superior to traditional tumor antigen binding sites, and has attractive clinical application prospects.

[0039] Single-chain antibody (scFv) is a small molecule peptide that connects the heavy chain variable region (VH) and light chain variable region (VL) by a connecting peptide, which maintains the specificity and affinity of natural antibodies, and can be genetically engineered...

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Abstract

The invention discloses a tumor vascular endothelial cell targeted IP10 single-chain antibody and a preparation method. The preparation method comprises the following steps: S1, synthesizing human-derived IP10 extracellular domain encoding genes depending on a gene pool; S2, constructing single-chain antibody genes S3 of cell membrane glycoprotein Endoglin by virtue of a recombinant phage antibody system; S3, cloning the IP10 and the single-chain antibody genes of Endoglin into a prokaryotic expression vector, and promoting linkage by virtue of a flexible joint so as to obtain encoding genes of the IP10 single-chain antibody; and S4, conducting expression in escherichia coli, so that the IP10 single-chain antibody is obtained. According to the IP10 single-chain antibody provided by the invention, IP10 protein at a high concentration can be obtained in a tumor microenvironment, and chemotaxis of targeted killing activity of T cells on tumor cells can be promoted; and by conducting adoptive therapy in combination with CIK cells in the clinical field, a killing effect of the CIK cells on the tumor cells can be enhanced, so that an anti-tumor effect is enhanced.

Description

technical field [0001] The invention belongs to the medical field, and in particular relates to a chemokine interferon-inducible protein 10 (IP10) single-chain antibody targeting tumor vascular endothelial cells, a preparation method, and an application in a drug for adopting CIK cells to treat tumors. Background technique [0002] Malignant tumors, as a class of diseases that seriously endanger human health, are one of the important causes of death from diseases worldwide. Among them, the incidence of liver cancer occupies the sixth place, and the death rate occupies the third place. Liver cancer develops rapidly and is easy to invade or metastasize. At present, clinical treatment mainly adopts methods such as surgical resection, radiotherapy and chemotherapy, and liver transplantation, but these methods are difficult to cure completely, and the treatment results are not satisfactory. Therefore, it is urgent to find a new treatment strategy. Immune cell adoptive therapy,...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/13C12N15/70C07K16/24A61K39/395A61K35/17A61P35/00
Inventor 卢小玲赵永祥
Owner GUANGXI MEDICAL UNIVERSITY
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