A kind of carbon quantum dot fluorescent labeling material and preparation method thereof

Abstract

The invention discloses a carbon quantum dot fluorescent label material and a preparation method thereof. According to the technical scheme, the method comprises the steps of adding coal-based pyrolytic carbon and concentrated HNO3 to a reaction kettle at the mass ratio of 1:(10-25), carrying out stirring and refluxing at 85-110 DEG C for 3-5h, cooling and filtering to obtain filtrate; adding a NaOH solution to the filtrate and adjusting the pH value to 5-9 to obtain brown black liquid; adding absolute ethyl alcohol to the brown black liquid at the volume ratio of 1:(3-5), and carrying out ultrasonic concussion and centrifugal separation to obtain a supernate and a submerged substance, wherein the submerged substance is a carbon quantum dot solution; and carrying out freeze drying on the carbon quantum dot solution at -50 DEG C to -60 DEG C for 24-48h to obtain the carbon quantum dot fluorescent label material. The carbon quantum dot fluorescent label material has the characteristics of being low in reaction temperature, simple in process, high in yield, low in cost and beneficial to industrial production, and the prepared carbon quantum dot fluorescent label material is high in quality.

Description

technical field [0001] The invention belongs to fluorescent marking materials. In particular, it relates to a carbon quantum dot fluorescent marking material and a preparation method thereof. Background technique [0002] Carbon quantum dots are a new member of the carbon nano family. They are a new type of fluorescent nanomaterial with a size below 10nm. They have excellent properties such as chemical inertness, good photostability, and good water solubility. Clemson University scientist Scrivens prepared it, which aroused great interest of researchers. Since the surface of carbon quantum dots contains a large number of carboxyl groups, it has good water solubility and is easy to modify the surface. It can be combined with organic, inorganic, polymeric and biological materials to achieve related functionalization. At the same time, the low toxicity of carbon quantum dots makes it have good biocompatibility, and has little interference with the activity of biomolecules. It...

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Problems solved by technology

[0004] "A preparation method of fluorescent carbon quantum dot probe for detecting beryllium in water" (CN104003370A) patented technology, the specific steps are to mix urea and citric acid and react at 200-240°C for 5-7 hours to obtain a brown color Aqueous solution, and then the brown aqueous solution is subjected to centrifugal dialysis, rotary evaporation, and vacuum drying to obtain the product. The preparation method of carbon quantum dots has harsh conditions and complicated operations, and the promotion is poor.

[0005] These quantum dot synthesis processes have complex procedures, expensive reagents, high reaction temperatures, difficult separations, and low yields.

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