Morphine detection method based on SPR technique

A detection method and morphine technology, which are used in measuring devices, material analysis by optical means, instruments, etc., to achieve the effect of convenient purchase and easy operation

Inactive Publication Date: 2017-06-27
ZHEJIANG POLICE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the combination of morphine detection and SPR technology at home and abroad

Method used

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  • Morphine detection method based on SPR technique
  • Morphine detection method based on SPR technique
  • Morphine detection method based on SPR technique

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Turn on the SPR instrument, unlock the SPR chip slot, place the SPR chip in the slot, and lock the chip slot; the temperature of the SPR instrument is set to 25°C;

[0042] (2) At a flow rate of 20 μL / min, inject 250 μL of a mixture of EDC and NHS in an equal volume ratio on the surface of the SPR chip to activate the carboxyl groups on the surface of the chip; the concentration of EDC is 0.4M, and the concentration of NHS is 0.1M;

[0043] (3) BSA-Morphine solution of 1 mg / mL pH5.0 is passed through the SPR chip after activating the surface carboxyl group at a flow rate of 20 μL / min, so that it is coupled to the activated carboxyl group, and the target response value (RU ) reaches 3000;

[0044] (4) Pass 100 μL of 1M ethanolamine hydrochloride solution at a flow rate of 20 μL / min on the SPR chip coupled with BSA-Morphine to block unreacted activated carboxyl sites;

[0045] (5) 40 μL of morphine antibody with gradient concentrations of 3.13 μg / mL, 6.25 μg / mL, 12.5...

Embodiment 2

[0053] (1) Turn on the SPR instrument, unlock the SPR chip slot, place the SPR chip in the slot, and lock the chip slot; the temperature of the SPR instrument is set to 25°C;

[0054] (2) Pass a mixture of 100 μL 0.2M EDC and 100 μL 0.1M NHS to the surface of the SPR chip at a flow rate of about 10 μL / min to activate the carboxyl groups on the surface of the chip;

[0055] (3) Pass 0.5mg / mLBSA-Morphine solution (pH value is 4.5) with the flow rate of 10μL / min on the SPR chip after the surface carboxyl group is activated in step (2), make it couple to the activated carboxyl group, require The target response value (RU) reached about 2000;

[0056] (4) Pass 75 μL of 2M ethanolamine hydrochloride solution at a flow rate of 10 μL / min on the SPR chip after BSA-Morphine coupling in step (3) to block unreacted activated carboxyl sites;

[0057] (5) At a flow rate of 10 μL / min, 30 μL of morphine antibody with gradient concentrations of 3.13 μg / mL, 6.25 μg / mL, 12.5 μg / mL, 25 μg / mL, 50...

Embodiment 3

[0065] (1) Turn on the SPR instrument, unlock the SPR chip slot, place the SPR chip in the slot, and lock the chip slot; set the temperature of the SPR instrument to 30°C;

[0066] (2) Pass a mixture of 150 μL 0.2M EDC and 150 μL 0.2M NHS to the surface of the SPR chip at a flow rate of about 30 μL / min to activate the carboxyl groups on the surface of the chip;

[0067] (3) Pass 2mg / mL BSA-Morphine solution (pH value is 5.5) with the flow velocity of 30 μ L / min on the SPR chip after the surface carboxyl group is activated in step (2), make it couple to the carboxyl group after activation, require The target response value (RU) reached about 3000;

[0068] (4) Pass 100 μL of 2M ethanolamine hydrochloride solution at a flow rate of 30 μL / min on the SPR chip after BSA-Morphine coupling in step (3) to block unreacted activated carboxyl sites;

[0069] (5) At a flow rate of 30 μL / min, 50 μL of morphine antibodies with gradient concentrations of 3.13 μg / mL, 6.25 μg / mL, 12.5 μg / mL, ...

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Abstract

The invention discloses a morphine detection method based on a SPR technique. The method comprises the following steps: coupling a SPR chip with an amino group on BSA through a EDC/NHS activated carboxyl group and then taking the SPR chip as a sensing chip, and when a to-be-detected sample passes by the surface of the sensing chip, utilizing a surface plasma resonance detector to detect the change in light intensity of the total reflected light at an interface of to-be-detected saliva and a molecular recognition sensitive chip so as to directly determine the morphine content in saliva. The method provided by the invention is high in detection sensitivity, is capable of greatly reducing the loss rate of drug addict detection and increasing the working efficiency and has an important application value.

Description

technical field [0001] The invention belongs to the field of drug detection and relates to a method for detecting morphine based on SPR technology. Background technique [0002] Morphine is purified from opium and is a white crystal with a content of about 10% in opium. It is a colorless or white crystalline powder. Sharp pain and cardiac asthma. It will produce euphoria after smoking, and it is more addictive than opium. Long-term use can cause mental disorders, delirium and hallucinations, and excessive use can lead to respiratory failure and death. Historically, it was used as a psychotropic drug for withdrawal from opium, but due to its excessive side effects, it was eventually designated as a drug. [0003] Although chemical methods, chromatography, spectroscopy, immunoassays, capillary electrophoresis and ion mobility spectrometry have been applied to drug detection, the sensitivity of chemical methods is low, and the pretreatment of chromatography, capillary electr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/552G01N30/02
CPCG01N21/553G01N30/02
Inventor 王继业孟凡伟
Owner ZHEJIANG POLICE COLLEGE
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