Selenium-rich yeast, preparing method and application

A technology of selenium-enriched yeast and Candida, applied in biochemical equipment and methods, applications, mutant preparation, etc., can solve the problems of limiting the application of other yeast strains, serious homogeneity of selenium-enriched products, etc., and improve digestion and utilization The effect of reducing the rate, reducing the amount of addition, and reducing the cost

Inactive Publication Date: 2017-06-30
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for higher amounts of enzymes produced from rice that have been genetically modified into better ways than previously possible due to its unique characteristics such as being highly effective at converting nitrogen fertilizers into useful compounds like methyl mercaptans (CH3SH) without losing any nutrients during processing steps downstream. Additionally, this process produces an abundance of certain types of proteins called selenomyitrinos containing very low levels of cysteines compared to traditional methods where crops were grown on their own flour instead of grain. These beneficial properties make these materials valuable sources of natural resources while reducing costs associated with producing them separately.

Problems solved by technology

This patents discusses how different types of plants have been identified by genetic analysis that help predict potential uses related metals like selenium. However, current ways involve extracting pure elemental selenium from these sources, making them difficult to be applied directly due to their low concentration compared to what they could potentially provide. To address this problem, new techniques involving converting specific forms of selenium called methylselenoferric acid ("MSA") -a type of vitamin found naturally in foodstuffs –to create stable compounds containing both selenium and hydrogen atoms, known as bioselenium, were proposed earlier but still lacked in practicality because previous attempts at producing MSAs did not result in any significant improvement over existing processes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation method of the selenium-enriched yeast of the present embodiment is as follows:

[0034] (1) Breeding of selenium-enriched strains

[0035] Cultivate C.ethanolica on the YEPD culture plate for 24-36 hours, pick a single colony into a 250ml Erlenmeyer flask containing 100ml YEPD liquid medium, 160-180rpm, shake and culture for 20-24 hours, and centrifuge and wash the bacterial liquid 3 times , make a single cell suspension, so that the cell concentration is 10 8 ~10 9 individual / mL. Take 0.1mL of the bacterial suspension and evenly spread it on a sterile petri dish, and place it on a clean bench to air dry to form a bacterial film. in N + Ion implantation was carried out in the implanter with an energy of 15KeV. Six gradient doses were made for each strain, with two plates for each dose. N + The injected dose was 2.0×10 14 , 4.0×10 14 , 6.0×10 14 , 8.0×10 14 , 1.0×10 15 , 1.2×10 15 ion / cm 2 , and make a vacuum control plate. After the mutage...

Embodiment 2

[0046] The preparation method of the selenium-enriched yeast of the present embodiment is as follows:

[0047] (1) Breeding of selenium-enriched strains

[0048] The process of selenium-enriched strain breeding is the same as in Example 1.

[0049] (2) Preparation of seed culture solution

[0050] Candida ethanol C.ethanolica cas8 was inoculated on the slant medium and cultured at 28°C for 20 hours to obtain the slant strain. The composition of the slant medium was: glucose 18g / L, peptone 17g / L, yeast extract 7g / L, sodium selenite 25mg / L, agar 14g / L.

[0051] The above slant strains were inoculated into 80 mL of seed culture medium, cultured with shaking at 28° C., 170 r / min, for 20 h, to obtain seed culture liquid. The composition of the seed medium is: malt extract 18g / L, peptone 13g / L, yeast extract 7g / L, sodium selenite 45mg / L.

[0052] (3) Fermentation culture

[0053] The composition of the fermentation medium is: fermentable reducing sugar 250g / L, copper sulfate 0....

Embodiment 3

[0059] The preparation method of the selenium-enriched yeast of the present embodiment is as follows:

[0060] (1) Breeding of selenium-enriched strains

[0061] The process of selenium-enriched strain breeding is the same as in Example 1.

[0062] (2) Preparation of seed culture solution

[0063] The above-mentioned Candida ethanol C.ethanolica cas8 was inoculated on the slant medium and cultured at 28°C for 24 hours to obtain the slant strain. The composition of the slant medium was: glucose 20g / L, peptone 20g / L, yeast extract 10g / L, selenous acid Sodium 30mg / L, agar 18g / L.

[0064] Inoculate the above-mentioned slant strains into 80 mL of seed culture medium, culture at 28° C., 180 r / min, and shake for 24 hours to obtain a seed culture solution. The composition of the seed medium is: malt extract 20g / L, peptone 15g / L, yeast extract 10g / L, sodium selenite 60mg / L.

[0065] (3) Fermentation culture

[0066] The composition of the fermentation medium is: fermentable reduci...

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PUM

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Abstract

The invention discloses a selenium-rich yeast, a preparing method and an application. The preparing method includes the following steps that Candida ethanolica is subjected to nitrogen ion-implantation mutagenesis, and is subjected to mutation screening for multiple times, and an Candida ethanolica mutant strain tolerating high-concentration sodium selenite is obtained; a seed culturing solution is prepared, the seed culturing solution is inoculated in a fermentation medium and fermented to the logarithmic phase, the sodium selenite is additionally added into a fermented product, the mixture continues to be fermented and cultured, and a fermented product is collected; fermented liquid is subjected to centrifugal washing and dried, and then a faint-yellow powdery product is obtained. The selenium-rich yeast has a large amount of amino acid and small peptide, and is extremely and easily digested and absorbed by animals, organic selenium in the yeast can be fully absorbed by living bodies along with absorption of the amino acid and the small peptide, and the digestion using rate of the organic selenium is greatly increased. Under the condition of the equivalent additive amount, the selenium-rich yeast has the advantage of being obvious in production performance aspect compared with similar products, or under the condition of the equivalent production performance, the additive amount can be reduced, and the cost is reduced.

Description

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Claims

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Application Information

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Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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