Selenium-rich yeast, preparing method and application
A technology of selenium-enriched yeast and Candida, applied in biochemical equipment and methods, applications, mutant preparation, etc., can solve the problems of limiting the application of other yeast strains, serious homogeneity of selenium-enriched products, etc., and improve digestion and utilization The effect of reducing the rate, reducing the amount of addition, and reducing the cost
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Embodiment 1
[0033] The preparation method of the selenium-enriched yeast of the present embodiment is as follows:
[0034] (1) Breeding of selenium-enriched strains
[0035] Cultivate C.ethanolica on the YEPD culture plate for 24-36 hours, pick a single colony into a 250ml Erlenmeyer flask containing 100ml YEPD liquid medium, 160-180rpm, shake and culture for 20-24 hours, and centrifuge and wash the bacterial liquid 3 times , make a single cell suspension, so that the cell concentration is 10 8 ~10 9 individual / mL. Take 0.1mL of the bacterial suspension and evenly spread it on a sterile petri dish, and place it on a clean bench to air dry to form a bacterial film. in N + Ion implantation was carried out in the implanter with an energy of 15KeV. Six gradient doses were made for each strain, with two plates for each dose. N + The injected dose was 2.0×10 14 , 4.0×10 14 , 6.0×10 14 , 8.0×10 14 , 1.0×10 15 , 1.2×10 15 ion / cm 2 , and make a vacuum control plate. After the mutage...
Embodiment 2
[0046] The preparation method of the selenium-enriched yeast of the present embodiment is as follows:
[0047] (1) Breeding of selenium-enriched strains
[0048] The process of selenium-enriched strain breeding is the same as in Example 1.
[0049] (2) Preparation of seed culture solution
[0050] Candida ethanol C.ethanolica cas8 was inoculated on the slant medium and cultured at 28°C for 20 hours to obtain the slant strain. The composition of the slant medium was: glucose 18g / L, peptone 17g / L, yeast extract 7g / L, sodium selenite 25mg / L, agar 14g / L.
[0051] The above slant strains were inoculated into 80 mL of seed culture medium, cultured with shaking at 28° C., 170 r / min, for 20 h, to obtain seed culture liquid. The composition of the seed medium is: malt extract 18g / L, peptone 13g / L, yeast extract 7g / L, sodium selenite 45mg / L.
[0052] (3) Fermentation culture
[0053] The composition of the fermentation medium is: fermentable reducing sugar 250g / L, copper sulfate 0....
Embodiment 3
[0059] The preparation method of the selenium-enriched yeast of the present embodiment is as follows:
[0060] (1) Breeding of selenium-enriched strains
[0061] The process of selenium-enriched strain breeding is the same as in Example 1.
[0062] (2) Preparation of seed culture solution
[0063] The above-mentioned Candida ethanol C.ethanolica cas8 was inoculated on the slant medium and cultured at 28°C for 24 hours to obtain the slant strain. The composition of the slant medium was: glucose 20g / L, peptone 20g / L, yeast extract 10g / L, selenous acid Sodium 30mg / L, agar 18g / L.
[0064] Inoculate the above-mentioned slant strains into 80 mL of seed culture medium, culture at 28° C., 180 r / min, and shake for 24 hours to obtain a seed culture solution. The composition of the seed medium is: malt extract 20g / L, peptone 15g / L, yeast extract 10g / L, sodium selenite 60mg / L.
[0065] (3) Fermentation culture
[0066] The composition of the fermentation medium is: fermentable reduci...
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