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A method for the detection of mycotoxins based on dtnb-labeled gold@silver core-shell nanorods

A mycotoxin and detection method technology, applied in the preparation of test samples, material excitation analysis, Raman scattering, etc., can solve the problems of low specificity, low sensitivity, expensive instruments, etc., and achieve a wide detection range and large Scattering cross section, effect of Raman signal stabilization

Active Publication Date: 2019-08-27
中科怡海高新技术发展江苏股份公司
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  • Application Information

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Problems solved by technology

At present, conventional detection methods for mycotoxins mainly include biological identification methods, but this method can only be used for qualitative detection, and the specificity is not strong, and the sensitivity is low; chemical analysis method (thin layer chromatography), the precision is poor , it is difficult to apply in practice; instrumental methods (gas chromatography, liquid chromatography, GC-MS, LC-MS), but the pretreatment is complex and the instruments are expensive; immunoassays (enzyme-linked immunosorbent assay, immunofluorescence technique , radioimmunoassay, etc.), but this method has some false positives

Method used

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  • A method for the detection of mycotoxins based on dtnb-labeled gold@silver core-shell nanorods
  • A method for the detection of mycotoxins based on dtnb-labeled gold@silver core-shell nanorods
  • A method for the detection of mycotoxins based on dtnb-labeled gold@silver core-shell nanorods

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Embodiment 1

[0026] In order to further verify the detection method prepared by the present invention to the detection of mycotoxins in food, examples of the present invention, taking aflatoxin B1 (AFB1) as an example, the specific operation steps are as follows:

[0027]Step 1) Preparation of gold@DTNB@silver core-shell nanorods: First, gold nanorods were synthesized by gold seed growth method, centrifuged and concentrated twice to remove excess CTAB, redispersed in deionized water, and 10 μL was added to every 10 mL of gold nanorod solution , 10mM DTNB ethanol solution, stirred magnetically at room temperature for 2h, centrifuged to remove unattached DTNB, and redispersed into the same volume of deionized water; 2mL of the gold@DTNB nanorods synthesized above was added to 4mL, 0.04mM CTAB solution under strong stirring After mixing evenly, add 150mL, 0.1M ascorbic acid, different volumes of 10mM silver nitrate, 250mL, 0.1M sodium hydroxide, and mix well. figure 1 TEM characterization dia...

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Abstract

The invention relates to a method for performing supersensitive detection on fungaltoxin of a DTNB mark-based gold@silver core-shell nanorod, and belongs to the technical fields of food safety, environment monitoring and the like. The gold nanorod is prepared by a seed growing method, 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) is marked on the gold nanorod, meanwhile, the gold nanorod is coated with a silver shell layer to prepare a gold@DTNB@silver surface Raman enhanced substrate, a superparamagnetic magnetic material chitosan ferroferric oxide is prepared, a fungaltoxin aptamer complementary chain is coupled on the prepared Raman enhanced substrate, a fungaltoxin aptamer chain is coupled on the magnetic material, an enhancer and the magnetic material are combined and a Raman signal of a system is strongest when the fungaltoxin does not exist in the detection system, and the aptamer modified magnetic material is specifically and preferentially combined with the fungaltoxin and the Raman signal of the system is changed after an external magnetic field is separated when the fungaltoxin exists, so that the aim of quantitatively detecting the fungaltoxin is fulfilled.

Description

technical field [0001] The invention relates to methods applicable to technical fields such as food safety and environmental monitoring, and in particular to a method for detecting mycotoxins based on DTNB-labeled gold@silver core-shell nanorods. Background technique [0002] Mycotoxins are common microbial toxins in grain foods such as corn and peanuts. They are toxic secondary metabolites produced by fungi growing in food or feed, and have the potential to cause liver cancer, gastric cancer, kidney cancer, and intestinal cancer. At present, conventional detection methods for mycotoxins mainly include biological identification methods, but this method can only be used for qualitative detection, and the specificity is not strong, and the sensitivity is low; chemical analysis method (thin layer chromatography), the precision is poor , it is difficult to apply in practice; instrumental methods (gas chromatography, liquid chromatography, GC-MS, LC-MS), but the pretreatment is c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65G01N1/28
Inventor 陈全胜杨明秀李欢欢欧阳琴郭志明孙浩刘妍
Owner 中科怡海高新技术发展江苏股份公司
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