Method for identifying cape jasmine variety by use of EST-SSR primers and application

Abstract

The invention provides a method for identifying cape jasmine variety by use of EST-SSR primers. The method comprises the following steps: extracting sample blade DNA of a test article variety, performing PCR amplification reaction, polyacrylamide gel electrophoresis and silver-staining and EST-SSR spectrum band analysis by use of the EST-SSR primer. The EST-SSR spectrum band analysis is performed after performing PCR amplification by use of 14 pairs of EST-SSR primers, thereby performing variety identification on the test cape jasmine variety and the known cape jasmine variety, and a constructed cape jasmine main cultivated variety EST-SSR fingerprint map can be used as a reference for cape jasmine variety identification. By use of the method for identifying cape jasmine variety by use of EST-SSR primers, the cape jasmine can be fast and efficiently identified, and the identification result is hardly influenced by the environment, the mainly cultivated varieties of the gardenia florida, the fructus gardeniae and the gardenia jasminoides which are planted in large area at present in different application types can be accurately identified through blade samples at the seedling stage of the cape jasmine.

Description

technical field [0001] The invention relates to the technical field of molecular biology molecular markers, in particular to a method and application for identifying gardenia varieties using EST-SSR primers. Background technique [0002] Gardenia jasminoides Ellis (Gardenia jasminoides Ellis) is an evergreen shrub of Rubiaceae (Gardenia) genus. Its fruit is used as medicine. Gardenia is a traditional bulk medicinal material in my country. It has been used for more than 2,000 years. It is mainly produced in Jiangxi and Hunan, and it is also commonly cultivated in Chongqing, Sichuan, Hubei, Zhejiang, Anhui, Fujian, Guangxi and other provinces and cities. At its peak, the artificial planting area of ​​Gardenia jasmine in Jiangxi Province exceeded 26,667 hectares, and it is one of the important local ecological and economic industries. Due to different growing environments, plus long-term artificial cultivation and breeding, some variations have occurred in its habits, shapes a...

AI Technical Summary

Problems solved by technology

Distinguishing gardenia varieties based on the appearance of flowers and fruits is limited by the age of the plants. It is difficult to distinguish effectively and reliably at the seedling stage of gardenia. Gardenia only blooms but does not bear fruit. Half, if you wait until the planted gardenias bloom and bear fruit, you will find that it is not the desired variety, which will inevitably cause great losses

At present, there are no reports on the method and application of EST-SSR molecular markers for distinguishing and identifying the main gardenia species

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