Bletilla glucomannan self-assembled nano particles, and preparation method and applications thereof

A technology of nano-particles and bletilla striata glue, which is applied in the field of medicine, can solve problems such as inability to rule out viral infection, difficult absorption, poor environmental stability, etc., and achieve the effect of improving hemostatic effect, good tissue adhesion, and good hemostatic performance

Active Publication Date: 2017-07-21
GUANGZHOU OUHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although animal fibrin glue products have the advantages of wide sources, low price, and avoiding human infectious diseases, they also have the following problems: ①The environmental stability is poor and low temperature storage is required; ②It is inconvenient to use and requires a certain amount of preparation time; Used as a drug carrier, such as in tumor resection, it cannot be used as a carrier for local chemotherapy; ④The biocompatibility of fibrin glue from animals is poor; possible
[0004] However, the molecular weight span of bletilla striata gum is very large, and the distribution ranges from 20 to 2500kDa. Although they all have hemostatic effect, the large molecular weight bletilla striata polysaccharide has relatively low activity and low stability, and is difficult to be absorbed by the human body. In vitro hemostasis

Method used

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  • Bletilla glucomannan self-assembled nano particles, and preparation method and applications thereof
  • Bletilla glucomannan self-assembled nano particles, and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Extraction and separation of bletilla striata gum:

[0036] (1) Take 300g of dried Bletilla striata tubers, crush them into small pieces of about 8 mesh, add 9000ml of purified water to soak at room temperature for 2 hours, heat and reflux for extraction for 2 hours, filter through a 40-mesh filter, and collect the filtrate; add 9000ml of purified water to the residue and soak at room temperature for 1 hour, Heat and reflux for extraction for 2 hours, filter through a 40-mesh filter, collect the filtrate, and combine the two extraction filtrates to obtain about 17200 ml of the first filtrate.

[0037] (2) Place the first filtrate above in a vacuum concentrator, and concentrate under reduced pressure at 70°C until it becomes thick, so that the content of the active ingredient glucomannan is about 30%, and about 400ml of the concentrated solution is obtained.

[0038] (3) Slowly add 143ml of 95% ethanol solution to the above concentrate, stir until the ethanol concentrati...

Embodiment 2

[0046] Extraction and separation of bletilla striata gum:

[0047] (1) Take 600g of dried Bletilla striata tubers, crush them into small pieces of about 6 mesh, add 12,000ml of purified water to soak at room temperature for 2 hours, heat and reflux for extraction for 2 hours, filter through a 40-mesh filter, and collect the filtrate; continue to add 10,000ml of purified water to soak at room temperature for 1 hour. Heat and reflux for extraction for 1.5 hours, filter through a 40-mesh filter, collect the filtrate, add 10,000 ml of purified water to the residue and soak at room temperature for 1 hour, heat and reflux for 1 hour, filter through a 40-mesh filter, collect the filtrate, and combine the extracted filtrates for 3 times to obtain the first filtrate of about 31200ml.

[0048] (2) Place the first filtrate above in a vacuum concentrator, and concentrate under reduced pressure at 65°C until it becomes thick, so that the content of the active ingredient glucomannan is abou...

Embodiment 3

[0057] Extraction and separation of bletilla striata gum:

[0058] (1) Take 300g of dried Bletilla striata tubers, crush them into small pieces of about 5 mesh, add 6000ml of purified water to soak at room temperature for 2 hours, heat and reflux to extract for 2 hours, filter through a 40 mesh filter, and collect the filtrate; continue to add 4500ml of purified water to soak at room temperature for 1 hour. Heated and refluxed for 1.5h, filtered through a 40-mesh filter, collected the filtrate, continued to add 4500ml of purified water to soak at room temperature for 0.5h, heated and refluxed for 1.0h, filtered through a 40-mesh filter, collected the filtrate, and combined the two extraction filtrates to obtain the first The filtrate is about 14100ml.

[0059] (2) Place the first filtrate above in a vacuum concentrator, and concentrate under reduced pressure at 75°C until it becomes thick, so that the content of the active ingredient glucomannan is about 28%, and about 430ml o...

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Abstract

The invention discloses bletilla glucomannan self-assembled nano particles, and a preparation method and applications thereof. According to the preparation method, bletilla glucomannan dry powder with a molecular weight ranging from 20 to 150kDa is extracted from bletilla striata tuber, and the bletilla glucomannan self-assembled nano particles are obtained via a plurality of steps. According to the bletilla glucomannan self-assembled nano particles, the porosity, the gelling capacity, and the stability of bletilla glucomannan are maintained and improved; no toxic or side effect is caused; the bletilla glucomannan self-assembled nano particles possess excellent biocompatibility, is capable of stopping bleeding, promoting wound healing, and stopping overflow of exudate, and can be used for tissue haemostasis, tissue sealing, and tissue bonding in surgical fields such as general surgery department, orthopedics department, cardiothoracic surgery department, neurosurgery department, and gynaecology and obstetrics.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to self-assembled nanoparticles of bletilla striata glue, which can be quickly used and has the functions of rapidly hemostasis, adhesion and wound sealing, and its preparation method and application. Background technique [0002] Excessive blood loss is the main cause of death from accidents and war injuries, and it is also one of the main causes of death during surgical treatment. Control of blood loss is a key link in surgery, first aid and field trauma care. Unfortunately, bleeding (bleeding) from various causes is very common, accidental injury bleeding (bleeding) in daily life and work, traumatic bleeding (bleeding) in surgery, traumatic bleeding (bleeding) from war, etc., If it cannot be controlled in time, it may cause huge losses or even death. Therefore, hemostasis has increasingly become a research hotspot in the medical field. Conventional hemostasis metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/08A61L24/00
CPCA61L24/001A61L24/08A61L2400/04A61L2400/12C08L5/00
Inventor 李颖陈惠良陈展权陈毅华陈桂恩陈桂柱
Owner GUANGZHOU OUHUA PHARMA
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