Application of vp1-aa 23-43 polypeptide derived from chicken infectious anemia virus as high-efficiency cell-penetrating peptide

A chicken infectious anemia and membrane-penetrating peptide technology, applied in the field of high-efficiency cell-penetrating peptides, can solve the problem of weakening the membrane-penetrating ability

Active Publication Date: 2019-12-03
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the TAT short peptide contains two Furin enzyme recognition cleavage sites (ie RKKR and RQRR), so TAT will be hydrolyzed by Furin during the membrane penetration process to destroy the TAT structure, thereby weakening its membrane penetration ability

Method used

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  • Application of vp1-aa 23-43 polypeptide derived from chicken infectious anemia virus as high-efficiency cell-penetrating peptide
  • Application of vp1-aa 23-43 polypeptide derived from chicken infectious anemia virus as high-efficiency cell-penetrating peptide
  • Application of vp1-aa 23-43 polypeptide derived from chicken infectious anemia virus as high-efficiency cell-penetrating peptide

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Embodiment Construction

[0026] In order to better understand the content of the present invention, the following embodiments illustrate the preparation and identification process of a high-efficiency cell-penetrating peptide derived from the chicken infectious anemia virus VP1-aa 23-43 polypeptide in conjunction with the accompanying drawings.

[0027] Implementation process

[0028] (1) Preparation and synthesis of short N-terminal peptide (aa 23-43) of chicken infectious anemia virus VP1

[0029] Using the online database UniProt (http: / / www.uniprot.org / uniprot / ), the N-terminus (aa 1-60) of the VP1 sequence from different CAV reference strains was compared, analyzed and marked in the sequence Positively charged polar amino acids (such as figure 1 ). According to the annotation information of the Family&Domains module, the N-terminal (aa1-60) sequence of the CAV VP1 sequence was characterized. It was found that the N-terminus of VP1 was highly conserved. According to the UniProt database inform...

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Abstract

The invention relates to the application of a VP1-aa 23-43 polypeptide derived from chicken infectious anemia virus (Chicken anemia virus, CAV) as a high-efficiency cell-penetrating peptide. The VP1-aa 23-43 polypeptide sequence is shown in SEQ ID NO.2. The peptide can carry FITC into 293T cells, HCT‑116 cells, 3T3 cells, MDCK cells, and MSB1 cells within 30 minutes, and the membrane penetration efficiency is positively correlated with the concentration of the membrane penetration peptide. The key feature is that it can efficiently enter suspension culture cells. Combining the results of laser confocal microscopy and flow cytometry, the chicken infectious anemia virus VP1‑aa 23‑43 polypeptide has a membrane penetration efficiency more than 2 times higher than that of the TAT short peptide at 10 μM.

Description

technical field [0001] The invention relates to a high-efficiency cell-penetrating peptide derived from chicken anemia virus (Chicken anemia virus, CAV) VP1-aa23-43 polypeptide. The polypeptide can carry FITC and penetrate into different cells (including adherent cells and suspension cells) within 30 minutes. Compared with TAT, it was found that the cell-penetrating function of the polypeptide to FITC was more than 2 times higher than that of TAT. Therefore, the high-efficiency cell-penetrating peptide derived from chicken infectious anemia virus VP1-aa 23-43 polypeptide obtained in the present invention has certain application prospects and value. Background technique [0002] Cell penetrating peptides (CPPs) are short peptides that can carry biomacromolecules into cells, generally 5-30 in length and rich in positively charged basic amino acids. It can not only efficiently pass through the cell membrane and enter cells, but also efficiently carry different types of exogen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/01
CPCC07K14/005C12N2750/10022
Inventor 叶建强呼高伟邵红霞秦爱建申秋平田晓彦金甫刘敏
Owner YANGZHOU UNIV
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