Application of vp1-aa 23-43 polypeptide derived from chicken infectious anemia virus as high-efficiency cell-penetrating peptide
A chicken infectious anemia and membrane-penetrating peptide technology, applied in the field of high-efficiency cell-penetrating peptides, can solve the problem of weakening the membrane-penetrating ability
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[0026] In order to better understand the content of the present invention, the following embodiments illustrate the preparation and identification process of a high-efficiency cell-penetrating peptide derived from the chicken infectious anemia virus VP1-aa 23-43 polypeptide in conjunction with the accompanying drawings.
[0027] Implementation process
[0028] (1) Preparation and synthesis of short N-terminal peptide (aa 23-43) of chicken infectious anemia virus VP1
[0029] Using the online database UniProt (http: / / www.uniprot.org / uniprot / ), the N-terminus (aa 1-60) of the VP1 sequence from different CAV reference strains was compared, analyzed and marked in the sequence Positively charged polar amino acids (such as figure 1 ). According to the annotation information of the Family&Domains module, the N-terminal (aa1-60) sequence of the CAV VP1 sequence was characterized. It was found that the N-terminus of VP1 was highly conserved. According to the UniProt database inform...
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