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Ixeris chinensis extractive and preparation method and organic acid component detecting method thereof

A kind of bitter root extract technology, which is applied in the field of Chinese bitter root extract, its preparation method and the content detection of phenolic components, can solve the problems of unsuitability for industrial production and high production cost, and achieve low cost, Good effect and simple process

Inactive Publication Date: 2017-08-11
SHANXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention overcomes the defects of high production cost and unsuitability for industrialized production in the existing traditional Chinese medicine extract preparation method, and provides a Chinese bitter root extract and a preparation method thereof

Method used

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  • Ixeris chinensis extractive and preparation method and organic acid component detecting method thereof
  • Ixeris chinensis extractive and preparation method and organic acid component detecting method thereof
  • Ixeris chinensis extractive and preparation method and organic acid component detecting method thereof

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Effect test

Embodiment 1

[0069] The preparation of embodiment 1 Chinese bitter root extract

[0070] Take 1 kg of dried medicinal material of the whole herb of Chia chinensis, crush it into 40 meshes, add 8 times the amount of 50% ethanol aqueous solution for reflux extraction once, each time for 1.0 hour, pour out the extract, add 10 times the amount of 50% ethanol aqueous solution for reflux extraction of the residue For 1 hour, combine all the extracts, concentrate under reduced pressure to 1 / 4 of the original volume, decolorize with activated carbon for 30 minutes, centrifuge at 4000r / min for 10 minutes, pour out the supernatant, add 2 times the volume of the supernatant to dilute with distilled water, and pass the diluted solution through Organic membrane filtration, membrane pore diameter 450nm, operating pressure 0.2MPa, operating temperature 25 ℃, obtain the organic membrane permeate, the filtrate after filtering through the filter membrane is carried out low-temperature drying, temperature 65 ...

Embodiment 2

[0071] The preparation of embodiment 2 Chinese bitter root extract

[0072] Take 1 kg of dried medicinal material of Chia chinensis, crush it into 40 meshes, add 10 times the amount of 70% ethanol aqueous solution for reflux extraction twice, each time for 1.0 hour, pour out the extract, add 10 times the amount of 70% ethanol aqueous solution for reflux extraction of the residue for 1 hour , combined all the extracts, concentrated under reduced pressure to 1 / 4 of the original volume, decolorized with activated carbon for 30 minutes, centrifuged at 4000r / min for 10 minutes, poured out the supernatant, added 2 times the volume of the supernatant to dilute with distilled water, and the diluted solution passed through the organic membrane Filtration, membrane pore diameter 450nm, operating pressure 0.2MPa, operating temperature 28 ° C, to obtain the organic membrane permeate, the filtrate after filtration through the filter membrane is dried at low temperature, the drying temperatu...

Embodiment 3

[0073] The preparation of embodiment 3 Chinese bitter root extract

[0074] Take 1 kg of dried medicinal material of Chia chinensis, crush it into 40 meshes, add 12 times the amount of 60% ethanol aqueous solution for reflux extraction once, each time for 1.5 hours, pour out the extract, add 10 times the amount of 60% ethanol aqueous solution for reflux extraction of the residue for 1 hour , combined all the extracts, concentrated under reduced pressure to 1 / 4 of the original volume, decolorized with activated carbon for 30 minutes, centrifuged at 4500r / min for 10 minutes, poured out the supernatant, added 2 times the volume of the supernatant to dilute with distilled water, and the diluted solution passed through the organic membrane Filtration, membrane pore diameter 450nm, operating pressure 0.2MPa, operating temperature 23 ° C, to obtain organic membrane permeate, the filtrate after filtration through the filter membrane is subjected to low-temperature drying, drying temper...

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Abstract

The invention discloses an ixeris chinensis extractive and an application thereof as a medicine for preventing liver injuries. The extractive comprises, by weight, 1.49-3.04% of caftaric acid, 6.14-11.43% of cichoric acid, 2.69-5.08% of chlorogenic acid, and 1.04-2.72% of 4,5-dicaffeoylquinic acid. The invention further discloses a preparation method and an organic acid component detecting method of the ixeris chinensis extractive. The preparation method of the ixeris chinensis extractive is simple in process, low in cost and energy consumption, and easy in industrialization.

Description

technical field [0001] The invention relates to a plant extract, a preparation method thereof, and a detection method for components thereof, in particular to a Chinese bitter gourd extract, a preparation method thereof, and a detection method for the content of phenolic components. Background technique [0002] Chinese bitter gourd is a medicinal plant of the family Asteraceae. Chinese bitter gourd is widely distributed in my country and has rich resources. It is used as a whole herb. The effect of quenching thirst is used for intestinal abscess, lung abscess, lung-heat cough, enteritis, dysentery, cholecystitis, pelvic inflammatory disease, boils, swollen poison, scrotum eczema, hematemesis, epistaxis, metrorrhagia, bruises, diabetes . The chemical composition of Chinese bitter root is complex, mainly containing sesquiterpenes, triterpenoids, adenosine, flavonoids, coumarins, etc., in addition to sterols, lignans, vitamins, amino acids and other compounds, currently At hom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P1/16G01N30/02G01N30/06
CPCA61K36/28A61K2236/15A61K2236/333A61K2236/39A61K2236/51A61K2236/53G01N30/02G01N30/06
Inventor 裴香萍裴妙荣刘海霞李慧峰
Owner SHANXI UNIV OF CHINESE MEDICINE
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