A kind of fermentation process of refined glutamic acid

A fermentation process, glutamic acid technology, applied in the field of refined glutamic acid fermentation process, can solve the problems of difficult wastewater treatment, large amount of acid and alkali, large amount of wastewater, etc., to achieve low consumption of sulfuric acid, good color and sense of finished products, The effect of increasing speed

Active Publication Date: 2020-09-11
内蒙古阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires a large amount of acid and alkali, a large amount of waste water, and high cost. Moreover, the waste water produced by the isoionization process is difficult to treat, and the problem of environmental pollution is very serious.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A fermentation process for refining glutamic acid, comprising the steps of:

[0028] Corynebacterium glutamicum (Corynebacterium glutamicum) ATCC13761 was inoculated into the seed medium, and at 32° C., 100 rpm shaker was cultivated for 12 hours to obtain the Corynebacterium glutamicum seed liquid; the group of the Corynebacterium glutamicum seed medium Divided into (the following are mass percentages): glucose 3%, corn steep liquor 3%, urea 1%, K 2 HPO 4 0.2%, Na 2 HPO 4 0.2%, manganese sulfate 0.01%, MgSO 4 0.01%, sterilized at 115°C for 15 minutes, and adjusted to pH 4-4.5;

[0029] The seed solution was inoculated into the fermenter with 8% inoculum, and the fermentation time was 48 hours; 0-46 hours, the temperature was controlled at 32°C and normal pressure, wherein, at the 40th hour, Triton X-100 (Triton X-100) was added -100), the addition amount is 1 μg / ml; at the 47th to 48th hour, the temperature is controlled at 39°C, and the pressure is 3 atmospheres, ...

Embodiment 2

[0033] A fermentation process for refining glutamic acid, comprising the steps of:

[0034] Corynebacterium glutamicum (Corynebacterium glutamicum) ATCC13761 was inoculated into the seed medium, and at 32° C., 100 rpm shaker was cultivated for 12 hours to obtain the Corynebacterium glutamicum seed liquid; the group of the Corynebacterium glutamicum seed medium Divided into (the following are mass percentages): glucose 3%, corn steep liquor 3%, urea 1%, K 2 HPO 4 0.2%, Na 2 HPO 4 0.2%, manganese sulfate 0.01%, MgSO 4 0.01%, sterilized at 115°C for 15 minutes, and adjusted to pH 4-4.5;

[0035]The seed solution was inoculated into the fermenter with 8% inoculum, and the fermentation time was 48 hours; 0-46 hours, the temperature was controlled at 32°C and normal pressure, wherein, at the 40th hour, Triton X-100 (Triton X-100) was added -100), the addition amount is 1-2μg / ml; at the 47th-48th hour, the control temperature is 40°C, and the pressure is 3.5 atmospheres, among w...

Embodiment 3

[0039] Effects of various factors on glutamic acid fermentation yield:

[0040] Set up a control group, wherein, control group 1: do not change the temperature and pressure, maintain the temperature at 32 ° C and under normal pressure conditions, the rest are the same as in Example 1; control group 2: do not add Triton X-100 and sodium chloride, All the other are with embodiment 1; Test group is embodiment 1. The production of glutamic acid in the fermented liquid of each group is shown in Table 1:

[0041] Table 1

[0042] group Control group 1 Control group 2 test group Glutamic acid production (g / L) 83.1 91.8 119.4

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Abstract

The invention belongs to the technical field of amino acid preparation and discloses a fermentation technology for refinement of glutamic acid. The technology comprises the steps as follows: step 1) preparation of a seed solution; step 2) preparation of a fermentation solution; step 3) separation; step 4) concentration and crystallization. With the adoption of the technology, the fermentation acid production efficiency is improved, and the purity and yield of a product obtained with the separation and purification technology are high.

Description

technical field [0001] The invention belongs to the technical field of amino acid preparation, and in particular relates to a fermentation process for refining glutamic acid. Background technique [0002] Glutamic acid is an acidic amino acid. The molecule contains two carboxyl groups, and its chemical name is α-aminoglutaric acid. Glutamic acid was discovered by Li Suxun in 1856. It is a colorless crystal with umami taste, slightly soluble in water, but soluble in hydrochloric acid solution, with an isoelectric point of 3.22. A large amount exists in cereal protein, and the content in animal brain is also more. Glutamic acid plays an important role in the protein metabolism process in organisms, and participates in many important chemical reactions in animals, plants and microorganisms. MSG is prepared from glutamic acid. [0003] Corynebacterium glutamicum is the main strain of microbial industrial fermentation to produce glutamic acid. People have been transforming s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/14C07C227/40C07C227/42C07C229/24C12R1/15
CPCC07C227/40C07C227/42C12P13/14C07C229/24
Inventor 刘海涛卢松伏广好李晓永许传娟高启超位风宇周敬刘苏宜
Owner 内蒙古阜丰生物科技有限公司
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