A method for preparing monosodium glutamate using isoelectric technology

An isoelectric and monosodium glutamate technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of difficult to fix process parameters, paralysis of fermentation system, bacterial contamination and other problems

Active Publication Date: 2020-07-07
内蒙古阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The applicant's previous patented technology "an environmentally friendly process for preparing monosodium glutamate by concentrated isoelectric process", this process uses two strains of compound fermentation, and prepares monosodium glutamate through concentrated isoelectric technology, its fermentation efficiency is high, environmental protection and pollution-free, but there are The risk of strain contamination increases. Once one of the strains is contaminated, the fermentation system will be paralyzed, and the process parameters of the two strains are not easy to fix, and need to be constantly explored and changed, resulting in increased operational difficulty

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method utilizing isoelectric technology to prepare monosodium glutamate, it comprises the steps:

[0024] Corynebacterium glutamicum (Corynebacterium glutamicum) ATCC13761 seed liquid (concentration is 1 × 10 9 CFU / mL) was inoculated into the fermenter with 10% (V / V) inoculum, and the fermentation time was 60 hours; 0-58 hours, the temperature was controlled at 32°C, normal pressure, and at the 50th hour, hexadecane was added Trimethylammonium bromide (CTAB), the addition amount is 3 μg / ml, wherein, at the 55th hour, add sodium chloride, the addition amount is 10 μg / ml; The pressure is 3 atmospheres; during the fermentation process, the pH is controlled at 4.5 by automatically feeding ammonia water; and the residual sugar is controlled at not less than 1.0 wt% by feeding a glucose solution with a concentration of 200g / L, and the fermentation is stopped at 60h to obtain fermentation liquid;

[0025] Among them, the fermentation medium is (mass percentage): glucose 6%...

Embodiment 2

[0029] A method utilizing isoelectric technology to prepare monosodium glutamate, it comprises the steps:

[0030] Corynebacterium glutamicum (Corynebacterium glutamicum) ATCC13761 seed liquid (concentration is 1 × 10 9 CFU / mL) was inoculated into the fermenter with 10% (V / V) inoculum, and the fermentation time was 60 hours; 0-58 hours, the temperature was controlled at 32°C, normal pressure, and at the 50th hour, hexadecane was added Trimethylammonium bromide (CTAB), the addition amount is 5 μg / ml; the 59th-60th hour, the control temperature is 40 ℃, the pressure is 3.5 atmospheres, wherein, at the 55th hour, add sodium chloride, the addition amount During the fermentation process, the pH is controlled at 4.5 by automatic feeding of ammonia water; and the residual sugar is controlled at not less than 1.0 wt% by feeding a glucose solution with a concentration of 200g / L, and the fermentation stops after 60 hours to obtain fermentation liquid;

[0031] Among them, the fermenta...

Embodiment 3

[0035] Effects of various factors on glutamic acid fermentation yield:

[0036] Set up a control group, wherein, control group 1: do not change the temperature and pressure, maintain the temperature at 32°C and under normal pressure conditions, the rest are the same as in Example 1; control group 2: do not add CTAB and sodium chloride, and the rest are the same as in Example 1 ; Test group is embodiment 1. The production of glutamic acid in the fermentation broth of each group is shown in Table 1:

[0037] Table 1

[0038] group Control group 1 Control group 2 test group Glutamic acid production (g / L) 90.5 98.3 124.6

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Abstract

The invention belongs to the technical field of biological fermentation, and discloses a method of preparing monosodium glutamate by utilizing an isoelectric technique. The method includes 1) a step of preparing a glutamic acid fermentation solution, 2) a step of performing centrifugation, ultrafiltration and concentration, 3) a step of performing isoelectric treatment and 4) a step of performing neutralization and crystallization. The method is simple, feasible, and suitable for large-scale production. The prepared monosodium glutamate is high in yield and purity.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, and specifically provides a method for preparing monosodium glutamate by using an isoelectric technology. Background technique [0002] Monosodium glutamate, the scientific name is sodium glutamate, and the chemical name is monosodium α-aminoglutaric acid. It is a salt formed by sodium ions and glutamate ions. Among them, glutamic acid is an amino acid and sodium is a metal element. . The main ingredient of seasoning MSG commonly used in life is sodium glutamate. Monosodium glutamate is a commonly used flavoring agent in daily life, which can increase the umami taste of food and help improve the digestibility of food by the human body. In addition, sodium glutamate has very important functions and is widely used in food, medicine, industry and agriculture. [0003] The applicant's previous patented technology "an environmentally friendly process for preparing monosodium glutama...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/14A23L27/24A23L27/22C12R1/15
CPCC12N1/20C12P13/14
Inventor 卢松伏广好许传娟王皓徐淑伟张建国李文全刘苏宜
Owner 内蒙古阜丰生物科技有限公司
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