Extraction and purification method of capsular polysaccharide in 336-type staphylococcus aureus

Abstract

The invention discloses an extraction and purification method of capsular polysaccharide in 336-type staphylococcus aureus. The method comprises the following steps: performing fermentation cultivation, inactivation and centrifugation on the 336-type staphylococcus aureus, extracting a capsular polysaccharide crude product from supernate and thallus sediments, deproteinizing the crude product and purifying the deproteinized crude product through exclusion chromatography with a molecular sieve, so as to obtain refined capsular polysaccharide. The method has the beneficial effects that liquid is adopted for fermentation cultivation, so that the process amplification and actual production application are realized easily; the polysaccharide is extracted from the supernate and the thallus sediments respectively, so that the production efficiency of the capsular polysaccharide is improved, and the production cost is lowered; deproteinization is realized by adopting a Sevage method, so that the dosage of phenol is reduced, the operation time is shortened, and the purification efficiency is improved; the yield of the polysaccharide is stable, so that the purified refined polysaccharide accords with the relevant national quality standard; the method is simple, convenient and efficient, the extracted capsular polysaccharide is suitable for diagnosis, prevention and treatment of diseases and is also suitable for preparation of capsular polysaccharide vaccines in the 336-type staphylococcus aureus.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to View More

AI Technical Summary

Benefits of technology

This patented technology allows for easier commercialization of microbial products like cell wall components called pectins that have potential medical applications. It also includes methods to improve their productivity without adding any foreign materials or other chemical substances during processing steps. Additionally, it simplifies the manufacturing processes while maintaining its effectiveness over long periods of storage.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the efficiency of producing VC protection against staphildup infections associated with various types of boviruses such as Streptospora sppiescences and Sarcoplasma necrotrophils. Current approaches involve isolating specific components within these organism through physical techniques like filtrations or enriching them separately before they could be applied clinically. These processes require multiple steps involving several laborious procedures, making their effectiveness challenging at scale up.

Images