GC-MS (Gas Chromatography-Mass Spectrometer) analysis method for quantitatively analyzing polycyclic aromatic hydrocarbon in aquatic product

Abstract

The invention relates to a polycyclic aromatic hydrocarbonanalytical test method in the field of analytical chemistry, and concretely relates to a gas chromatography-mass spectrometry method applicable to quantitatively analyzing polycyclic aromatic hydrocarbon in an aquatic product. Aiming at a complex matrix of an animal-origin (aquatic product) food rich in fat, protein and macromolecular substances, the method comprises the steps of utilizing gel permeation chromatography for degreasing, then utilizing an aluminium oxide-silica gel compound chromatography purification column for effectively extracting the polycyclic aromatic hydrocarbon, utilizing gas chromatography and mass spectrometry for carrying out quantitative analysis, and building the GC-MS (Gas Chromatography-Mass Spectrometer) analysis method for quantitatively analyzing the polycyclic aromatic hydrocarbon in the aquatic product. Compared with an existing method, the method provided by the invention is higher in target compound recovery rate, beneficial for removing matrix interference, improving detection limit and reducing detection cost, and applicable to aquatic product safety evaluation, and the like.

Description

technical field [0001] The invention relates to a GC-MS analysis method for quantitative analysis of polycyclic aromatic hydrocarbons in aquatic products, in particular to GC / MS analysis for the quantitative detection of polycyclic aromatic hydrocarbons in aquatic products by Soxhlet extraction, gel purification, and alumina-silica gel composite purification method. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are hydrocarbons formed by linking two or more aromatic rings, which belong to typical POPs and are widely distributed in natural environmental media. PAHs are mostly light yellow crystalline substances, not only have high melting point and boiling point, but also high octanol-water partition coefficient; but their vapor pressure and water solubility are low. There are many kinds of PAHs, and more than 400 kinds of monomers and their derivatives have been discovered so far, and most of them have strong carcinogenic, teratogenic, mutagenic and ...

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems of complicated pretreatment and poor detection effect of polycyclic aromatic hydrocarbons in complex environments existing in the prior art, the present invention provides a simple operation, less susceptible to magazine interference, accurate detection results, and capable of simultaneously detecting 16 kinds of aquatic products Polycyclic aromatic hydrocarbons (naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzene and[a]pyrene, dibenzo[a,h]anthracene, indeno[1,2,3-cd]pyrene, benzo[g,h,i]perylene) content method

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