A kind of preparation method and application of acid orange II standard substance

Abstract

The invention discloses a preparation method of a reference matter of acid orange II. The preparation method comprises the following steps of using an ethanol-water solution with volume ratio of (2 to 3):1 as a solvent, dissolving the raw material of acid orange II at the temperature of 80 to 100 DEG C, adding an ethanol-water solution with volume ratio of 1:1 by batches, continuously stirring, and continuing to perform heat preservation at the temperature of 95 to 105 DEG C; after heat filtering, cooling filtrate to room temperature, recrystallizing at the temperature of 4 DEG C, filtering a crystallizing body, drying at the temperature of 105 DEG C, grinding, screening, uniformly mixing, and drying, so as to obtain the reference matter of the acid orange II. The preparation method has the advantages that the prepared reference matter is used for studying a reference matter system, the domestic blank is filled, a quantity transfer system of the measuring process is established, and the important technical support is provided for the food safety detection and supervision management; the uncertainty of the prepared acid orange II reference matter is 1%; the acid orange II reference matter can be used for detecting acid orange II in the systems of foods or cosmetics or medicines and the like, and the accuracy and comparability of detection results are ensured.

Description

technical field [0001] The invention relates to a preparation method and application of acid orange II standard substance. Background technique [0002] People are good at using pigments to improve the sensory quality of food. Bright colors not only give people visual pleasure, but also increase appetite. However, some non-edible pigments have begun to flow into food because of their low price, strong coloring power, and good stability. Production line, in recent years, there have been constant turmoil of food safety issues, frequent occurrence of chemical additives exceeding the standard or quality problems, resulting in heavy casualties and property losses, endangering people's physical and mental health, making food safety issues increasingly become a hot spot of concern to the whole society Therefore, preventing food contamination and controlling food quality are important measures to prevent diseases and protect people's health. [0003] For the detection of the conten...

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Problems solved by technology

[0002] People are good at using pigments to improve the sensory quality of food. Bright colors not only give people visual pleasure, but also increase appetite. However, some non-edible pigments have begun to flow into food because of their low price, strong coloring power, and good stability. Production line, in recent years, there have been constant turmoil of food safety issues, frequent occurrence of chemical additives exceeding the standard or quality problems, resulting in heavy casualties and property losses, endangering people's physical and mental health, making food safety issues increasingly become a hot spot of concern to the whole society Therefore, preventing food contamination and controlling food quality are important measures to prevent diseases and protect people's health

[0003] For the detection of the content of a certain substance in a certain sample, a standard substance is often required as a control. At present, my country has also developed standard substances for the detection of food pigments (such as sunset yellow, amaranth, tartrazine, carmine, and allura red). However, the development of reference materials for Acid Orange II prohibited colorants is still in a blank stage

Most of the domestic food testing industry uses relevant foreign standard products. These standard products are not only expensive, but also have a long procurement cycle, and most of them are not traceable, which makes it impossible to effectively verify the testing methods and arbitrate the test results.

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