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Water body dissolved oxygen concentration measuring method based on time domain fluorescence lifetime technology

A technology of dissolved oxygen concentration and fluorescence lifetime, which is applied in the fields of ocean, resource and environment, can solve the problems of long phase locking period, demanding excitation light source, complex phase detection circuit, etc., achieve simple and easy implementation of detection circuit, and overcome the requirements of excitation light source Harsh, measurement stable and reliable effects

Inactive Publication Date: 2017-09-12
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Problems solved by technology

However, the detection method of dissolved oxygen concentration in water by the frequency-domain fluorescence lifetime method has strict requirements on the excitation light source, requires complex phase detection circuits, and has problems such as a long phase locking period.

Method used

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  • Water body dissolved oxygen concentration measuring method based on time domain fluorescence lifetime technology
  • Water body dissolved oxygen concentration measuring method based on time domain fluorescence lifetime technology
  • Water body dissolved oxygen concentration measuring method based on time domain fluorescence lifetime technology

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Embodiment Construction

[0027] A method for measuring dissolved oxygen concentration in water based on time-domain fluorescence lifetime technology, comprising the following steps:

[0028] (1) if figure 1 As shown, the oxygen-sensitive fluorescent film (fluorescent substance) is excited by a short pulse of light, and the fluorescent substance atoms jump to a high-energy state after being excited, and return to the ground state in the form of fluorescence emission, t 0 After the excitation light source is turned off at all times, the fluorescence luminous intensity decays exponentially with time, as shown in formula (1):

[0029]

[0030] Among them, I m is the initial value of the fluorescence intensity; τ is the fluorescence lifetime, which is the luminous intensity from the initial value I m The time required to reach its 1 / e;

[0031] (2) Select t on the fluorescence quenching curve 1 and t 1 ’Two moments, measure the fluorescence intensity A within the time interval Δ between two time po...

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Abstract

The invention discloses a water body dissolved oxygen concentration measuring method based on a time domain fluorescence lifetime technology. The fluorescence lifetime of an oxygen sensitive fluorescent screen is directly reversed in a time domain by using the two-point fluorescence intensity of a fluorescence quenching curve, and the dissolved oxygen concentration is obtained. According to the method, the stability of a measuring result is improved, and the influences of natural lighting and detector dark current on the measuring result are avoided. According to the method, the problems that the requirement of an excitation light source is strict, a phase detection circuit is complex and the stable period of phase difference is long in a frequency domain fluorescence lifetime measuring method are effectively overcome.

Description

technical field [0001] The invention belongs to the fields of resources, environment and ocean, in particular to a water body dissolved oxygen concentration measurement method based on time-domain fluorescence lifetime technology. Background technique [0002] Dissolved oxygen in water refers to the molecular oxygen dissolved in water, which is an indispensable condition for the survival of aquatic organisms and an important parameter to measure aquatic ecosystems. The level of dissolved oxygen can reflect water pollution, especially the degree of organic pollution, so it is also an important comprehensive evaluation index of water quality. Accurate and rapid acquisition of dissolved oxygen concentration information in water bodies is crucial to mastering the ecological environment of water bodies, ensuring drinking water safety, and developing aquaculture. [0003] At present, the commonly used dissolved oxygen detection methods mainly include iodometric method, electrode ...

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 殷高方赵南京涂梦迪覃志松甘婷婷方丽孟德硕段静波杨瑞芳肖雪马明俊刘建国刘文清
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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