Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for determining the fingerprint of a traditional Chinese medicine composition

A determination method and a technology of fingerprints, which are applied in the field of determination of fingerprints of traditional Chinese medicine compositions

Active Publication Date: 2021-05-04
SHIJIAZHUANG YILING PHARMA
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention claims to protect a high performance liquid chromatography fingerprint determination method of a traditional Chinese medicine composition, which is composed of the following medicinal materials: forsythia, honeysuckle, isatis root, bitter almond, menthol, Houttuynia cordata, rhubarb, patchouli , Mianma Guanzhong, Rhodiola rosea, ephedra, licorice, and gypsum. This fingerprint determination method calibrated 15 main chromatographic peaks, and identified 9 of them, which were neochlorogenic acid, chlorogenic acid, and cryptochlorogenic acid. , isoforsythiaside A, forsythiaside A, forsythin, quercetin, 4,5-di-O-caffeoylquinic acid, forsythin, glycyrrhizic acid, and there is no such thing in the prior art disclose the technical content

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for determining the fingerprint of a traditional Chinese medicine composition
  • A method for determining the fingerprint of a traditional Chinese medicine composition
  • A method for determining the fingerprint of a traditional Chinese medicine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0134] Weighing in proportion: Forsythia 200g, honeysuckle 300g, Radix Radix 200g, Rhubarb 40g, Patchouli 60g, Mianma Guanzhong 300g, Rhodiola rosea 100g, Menthol 9g, Ephedra 60g, Bitter almond 100g, Houttuynia cordata 200g, Licorice 100g, gypsum 200g, extract according to the following process:

[0135] (1) Weigh the Chinese herbal medicine according to the weight ratio of the raw material drug, select it cleanly, and break it as appropriate;

[0136] (2) Patchouli is broken, add 10 times the amount of water to extract the volatile oil, the oil extraction time is 8 hours, collect the volatile oil, and set aside; after the extract is filtered, the residue is discarded, and the filtrate is set aside;

[0137] (3) Forsythia, ephedra, houttuynia cordata, rhubarb, extract 3 times with 12 times the amount of 70% ethanol, each time for 2.5 hours, the extracts are combined and filtered, the ethanol is recovered, and the filtrate is used for later use;

[0138] (4) Honeysuckle, gypsu...

Embodiment 2

[0152] Weighing in proportion: Forsythia 300g, honeysuckle 200g, Radix Radix 300g, Rhubarb 60g, Patchouli 100g, Mianma Guanzhong 200g, Rhodiola rosea 60g, Menthol 5g, Ephedra 100g, Bitter almond 60g, Houttuynia cordata 300g, Licorice 60g, gypsum 300g, extract according to the following process:

[0153] (1) Weigh the Chinese herbal medicine according to the weight ratio of the raw material drug, select it cleanly, and break it as appropriate;

[0154] (2) Patchouli is broken, add 10 times the amount of water to extract the volatile oil, the oil extraction time is 8 hours, collect the volatile oil, and set aside; after the extract is filtered, the residue is discarded, and the filtrate is set aside;

[0155] (3) Forsythia, ephedra, houttuynia cordata, rhubarb, extract 3 times with 12 times the amount of 70% ethanol, each time for 2.5 hours, the extracts are combined and filtered, the ethanol is recovered, and the filtrate is used for later use;

[0156] (4) Honeysuckle, gypsum...

Embodiment 3

[0170] The formula of the raw material medicine is: 278g forsythia, 294g honeysuckle, 285g isatidis, 55g rhubarb, 95g patchouli, 290g Mianmaguanzhong, 87g rhodiola, 8.5g menthol, 88g ephedra, 80g bitter almond, 284g Houttuynia cordata , licorice 95g, gypsum 277g, extracted according to the following process:

[0171] (1) Weigh the Chinese herbal medicine according to the weight ratio of the raw material drug, select it cleanly, and break it as appropriate;

[0172] (2) Patchouli is broken, add 10 times the amount of water to extract the volatile oil, the oil extraction time is 8 hours, collect the volatile oil, and set aside; after the extract is filtered, the residue is discarded, and the filtrate is set aside;

[0173] (3) Forsythia, ephedra, houttuynia cordata, rhubarb, extract 3 times with 12 times the amount of 70% ethanol, each time for 2.5 hours, the extracts are combined and filtered, the ethanol is recovered, and the filtrate is used for later use;

[0174] (4) Honey...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
lengthaaaaaaaaaa
Login to View More

Abstract

The present invention requires the measurement method of high -efficiency liquid color spectrum fingerprint diagram of a traditional Chinese medicine composition. The Chinese medicine composition consists of the following medicinal materials: forsythia, honeysuckle, plate blue root, bitter almonds, mint brain, Houttuynia cordata, rhubarb, patchouli fragrance, Mianma Guanzhong, Hongjingtian, ephedra, licorice, gypsum, this fingerprint diagram is measured, and 15 major color spectrum peaks are calibrated, and 9 of them are identified, which are new green original acid, green original acid, hidden green original acidic acid acid, and hidden green acid acidic acid acid.Alcohol -glycoside A, Forsythin A, Forsythyl, Curchidin, 4,5‑ ‑ 啡 啡 oltyic acid, glycyrrhic acid.

Description

technical field [0001] The invention relates to a method for measuring the fingerprint of a traditional Chinese medicine composition. Background technique [0002] Fingerprint of traditional Chinese medicine refers to the chromatogram or spectrum that can mark the chemical characteristics of some traditional Chinese medicinal materials or preparations after proper treatment and certain analysis methods. Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification. It is based on the systematic research on the chemical components of traditional Chinese medicine and is mainly used to evaluate the authenticity, goodness and stability of the quality of Chinese medicinal materials and semi-finished products of traditional Chinese medicine preparations. "Integrity" and "ambiguity" are its salient features. It has become an international consensus to use fingerprints as a quality control method for extracts and preparations of traditional Ch...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 毕丹陈育鹏赵倩叶玉廷张水英任晋魏峰
Owner SHIJIAZHUANG YILING PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com