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A kind of detection method of botanical feedstuff polyoxin

A polyoxin, plant-derived technology, applied in the detection field of plant-derived feed polyoxin, can solve the problems of time-consuming, poor precision, low sensitivity, etc., to achieve accurate positioning, high identification accuracy, The effect of good application prospects

Active Publication Date: 2020-11-24
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these assay methods are used to determine a series of polyoxin metabolites produced by fermentation, which have low sensitivity, long time consumption and poor precision.

Method used

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  • A kind of detection method of botanical feedstuff polyoxin
  • A kind of detection method of botanical feedstuff polyoxin
  • A kind of detection method of botanical feedstuff polyoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take plant-derived feed from Hebei, Shandong, Fujian and other places:

[0023] (1) Pre-treatment of materials: Take 500g representative feed sample, reduce it by quarter method to at least 100g, grind, pass through a 0.9mm pore sample sieve, mix well and put it in a closed container, keep it in dark and low temperature for future use;

[0024] (2) Extraction and purification

[0025] Extraction: Weigh 2.5g sample, put it in a 50.0mL centrifuge tube, add 20mL 4% formic acid acetonitrile solution, vortex and mix for 1min, place it in ultrasonic extraction for 30min, then centrifuge at 5000r / min for 5min, take 8mL supernatant, Blow the nitrogen analyzer to nearly dry at 50°C, dissolve the extracted sample with 1mL of 2.5% ammonia solution and vortex for 30s, to be purified;

[0026] Purification: After pre-rinsing the mixed cation solid phase extraction column with 3.0mL acetonitrile and water in sequence, transfer the above sample to be purified into the column, discard the load...

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Abstract

The invention relates to a method for detecting polyoxin in plant-source feed. The method includes: preprocessing materials; extracting and purifying; performing qualitative assay determination; performing quantitative determination; performing result calculation and verification. The method is high in detection accuracy, accurate in positioning, representative in polyoxin detection of China and promising in application prospect.

Description

Technical field [0001] The invention belongs to the field of pesticide residue detection, and particularly relates to a detection method for plant-derived feed polyoxin. Background technique [0002] In recent years, with the prohibition of high-toxicity and high-residue chemical pesticides, green environmental protection, low-toxicity and low-residue biological pesticides have become more and more popular among farmers. Among them, polyoxin has the effect of inhibiting spore production and disease spot expansion. It is widely used to control cucumber downy mildew, wheat powdery mildew, corn smut, ginseng black spot, apple and pear gray spot and rice sheath blight Disease and many other diseases. However, the current studies on the toxicity of polyoxin to humans and animals are relatively lagging, and the toxicological effects are still unclear. However, rice, corn, wheat and other crops contaminated with polyoxin are used as feed and eaten by pigs, cattle and sheep. They will ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 曲明清王伟民李玉博宋卫国董茂峰周昌艳
Owner SHANGHAI ACAD OF AGRI SCI