A kind of melon anther induction medium and method for cultivating flowers
A technology for inducing culture medium and melon flowers, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problem of low haploid plants, improve the induction efficiency, increase the induction rate of melon anther callus, The effect of high industrial promotion value
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Embodiment 1
[0046] A melon anther induction medium, prepared according to the following formula:
[0047] The contents of the following components per liter of distilled water are: KNO 3 85mg / L, MgSO 4 ∙7H 2 O 150mg / L, KH 2 PO 4 73mg / L, Ca(NO 3 ) 2 ∙4H 2 O 180mg / L, MnSO 4 ∙4H 2 O 27.5mg / L, Na 2 -EDTA 12.5mg / L, FeSO 4 ∙7H 2 O13.5mg / L, H 3 BO 3 8.25mg / L, ZnSO 4 ∙7H 2 O 9.0mg / L, KI 0.5mg / L, AgNO 3 17mg / L, Na 2 MoO 4 ∙2H 2 O0.25mg / L, CuSO 4 ∙5H 2 O 0.025mg / L, CoCl 2 ∙6H 2 O 0.025mg / L, adenine 15mg / L, glutamine 500mg / L, vitamin B1 1.0mg / L, vitamin B6 0.7mg / L, Vc 0.6mg / L, niacin 5.0mg / L, inositol 130mg / L L, glycine 4.0mg / L, insulin 2.0mg / L, cyclic guanylic acid 0.2g / L, glutathione 32.5mg / L, ethyl methanesulfonate 1.2g / L, sucrose 45g / L, agar 7.0 g / L, 6-BA 1.0mg / L, NAA 1.0mg / L, mannitol 15g / L, coconut milk 30g / L, phytosulfokinetin PSK-α 20pmol / L, the optimal pH value of the preparation is 5.6.
[0048] After the medium is prepared, it is divided into 250ml Erlenmeyer f...
Embodiment 2
[0056] In the research and development process of muskmelon anther induction culture of the present invention, carried out the single-factor ratio test of medium component, the single-factor test component kind of medium formula is respectively: adenine, Vc, insulin, cyclic guanylic acid , ethyl methanesulfonate, coconut milk and phytosulfokinetin PSK-α. Each test component difference is only one, and other components are the same as in Example 1. The test melon varieties were also selected as Hongcheng No. 10 and Longtian No. 1. The preparation method of the medium and the method of flower cultivation were the same as in Example 1.
[0057] The specific ratios and test results of the single factor ratio test are shown in Table 1-Table 7 below.
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[0065] Can be found by the single factor test that the melon anther induction medium composition of table 1 to table 7 changes, the increase of insul...
Embodiment 3
[0067] In order to compare the difference between the muskmelon anther induction medium provided by the present invention and other media on the induction efficiency of muskmelon anther embryogenic callus, we selected two additional anther induction medium, and the test varieties also used Hongcheng No. 10 and Long For Tian No. 1, the anther isolation method, tissue culture operation method, and culture method are all the same as in Example 1. After the embryogenic callus is formed, the anthers of Hongcheng No. 10 and Longtian No. 1 are counted respectively in the two culture media. Anther callus induction rate.
[0068] The other 2 kinds of anther induction medium formulations are:
[0069] C17-2 medium: C17-2 basic medium + 20g / L sucrose + 7g / L agar + 2mg / L 2,4-D + 1mg / L kinetin, pH 5.8 (the reference file comes from F∙S∙ Wen et al. "Sorghum Anther and Inflorescence Culture Induced Callus and Regenerated Plants");
[0070] MS medium: MS basic medium + 3% sucrose + 1.0mg / LN...
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