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A method for detection and determination of aroma components that improve the sensory score of smoke

A technology for aroma components and detection methods, applied in the field of analytical chemistry, can solve the problems of cumbersome operation, long separation time, loss of phenolic substances or deterioration by oxidation, etc., and achieve the effects of reducing interfering ions, simple processing steps, and improved selectivity

Active Publication Date: 2019-01-08
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There have been many reports on the determination of phenolic substances in different matrices and samples. The pretreatment techniques include liquid-liquid extraction, column chromatography, derivatization, etc. The pretreatment process is complicated and tedious, and it is easy to cause the loss or oxidation of phenolic substances; Commonly used methods for the determination of phenolic substances include gas chromatography-mass spectrometry and high performance liquid chromatography. Gas chromatography-mass spectrometry is used to determine phenolic substances that are easily oxidized or decomposed by heat. Derivatization treatment is usually required, and the operation is very cumbersome, which affects the determination. The accuracy of the results, when measured by conventional high performance liquid chromatography, the separation time is long, and the pretreatment process is cumbersome
At present, there is no report on the determination method of 5-methylguaiacol in saliva, and the content of 5-methylguaiacol in saliva is low. - Determination method of methylguaiacol

Method used

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  • A method for detection and determination of aroma components that improve the sensory score of smoke
  • A method for detection and determination of aroma components that improve the sensory score of smoke
  • A method for detection and determination of aroma components that improve the sensory score of smoke

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Experimental program
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Effect test

Embodiment 1

[0020] Use a saliva collection tube to collect 2 mL of saliva from smokers. After centrifugation, take 1 mL of the supernatant and analyze it by ultra-high performance liquid chromatography-tandem mass spectrometry. Guaiacol content.

[0021] The described liquid chromatography conditions are as follows: the chromatographic column is selected from Waters ACQUITY UPLC HSS T3 post (1.8 μm, 2.1 × 100 mm) or equivalent column, the mobile phase is selected from acetonitrile / 0.5% acetic acid aqueous solution=15 / 85, and the column temperature is 39 °C, flow rate 0.6mL / min, injection volume 5μL.

[0022] The mass spectrometry conditions are as follows: ion source: electrospray ionization source (ESI), scan mode: positive ion scan, detection mode: multiple reaction monitoring (MRM), electrospray voltage: 4500 V, ion source temperature: 550 °C, auxiliary Gas1 pressure: 0.306MPa, auxiliary gas Gas2 pressure: 0.416 MPa; selected ion monitoring mode, ion selection parameters: 5-methylguai...

Embodiment 2

[0028] Collect 1mL of saliva from smokers with a saliva collection tube. After centrifugation, take 0.5mL of the supernatant and analyze it by ultra-high performance liquid chromatography-tandem mass spectrometry. Based guaiacol content.

[0029] The described liquid chromatography condition is as follows: the chromatographic column selects Waters ACQUITY UPLC HSS T3 post (1.8 μ m, 2.1 * 100 mm) or equivalent column, mobile phase selects the acetic acid aqueous solution=15 / 85 of acetonitrile / 1.0%, column temperature 30 °C, flow rate 0.1mL / min, injection volume 1μL.

[0030] The mass spectrometry conditions are as follows: ion source: electrospray ionization source (ESI), scan mode: positive ion scan, detection mode: multiple reaction monitoring (MRM), electrospray voltage: 4000 V, ion source temperature: 400 °C, auxiliary Gas1 pressure: 0.215MPa, auxiliary gas Gas2 pressure: 0.325MPa; selected ion monitoring mode, ion selection parameters: 5-methylguaiacol quantitative ion pa...

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Abstract

The invention relates to detection and judgment methods for an aroma component capable of increasing a smoke sense score, and belongs to the technical field of analytical chemistry. The related detection method is to measure the content of 5-methylguaiacol in saliva of a smoker through ultra-high performance liquid chromatography-tandem mass spectrometry. According to the method, a saliva collection pipe is used for collecting 1 to 2 mL of the saliva of the smoker; after centrifugation is completed, 0.5 to 1 mL of supernate is extracted, then is analyzed through an ultra-high performance liquid chromatography-tandem mass spectrometer and is quantified through an external standard method. The related judgment method is to analyze a relation between the content of the 5-methylguaiacol in the saliva of the smoker and a sense evaluation result. The detection method disclosed by the invention can be used for measuring the content of the 5-methylguaiacol in the saliva of the smoker, has the advantages of simple sample pretreatment, simplicity and convenience in operation, accurate quantification and the like, and is easy to popularize and apply.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a method for detecting and judging aroma components that improve the sensory score of smoke. Background technique [0002] Phenolic compounds are an important class of substances in cigarette smoke, which are closely related to the sensory quality of tobacco. Some of them have specific aroma, while others add a strange taste to the smoke and are not easily alleviated by other aromatizers. Phenolic substances have two sides in smoke. On the one hand, low-level phenols such as: phenol, hydroquinone, cresol, etc. have special irritating odors, especially strong irritation and corrosion to eyes, respiratory mucosa, skin, etc. effect. On the other hand, it is also believed that phenolic substances have a good effect on tobacco aroma, such as: p-cresol has astringent taste, 2,6-dimethylphenol has sweet taste, 4-ethylguaiacol has sweet and Vanilla, eugenol has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张凤梅刘志华刘春波尤俊衡唐石云司晓喜何沛申钦鹏朱瑞芝李振杰杨光宇王昆淼王晋苏钟璧蒋薇
Owner CHINA TOBACCO YUNNAN IND
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