Preparation method of taurine-enriched bacterial peptide feed produced by multi-strain fermentation of sesame meal
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A technology of multi-strain fermentation and sesame meal, applied in the field of agriculture and animal husbandry, can solve problems such as slow growth of fish, achieve normal visual function, maintain the balance of osmotic pressure of the body, and increase the effect of flavor
Inactive Publication Date: 2017-09-22
CHANGSHA UNIVERSITY
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Studies have shown that plant-based protein baits lacking taurine can lead to slow growth and some pathological phenomena in fish, such as green liver syndrome and hemolytic anemia
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Embodiment 1
[0019] Example 1: multi-strain fermentation to produce bacteriocin rich in taurine The feed contains the following raw materials in parts by mass: 9 parts of sesame meal, 0.5 part of bran, 0.3 part of molasses, 0.1 part of dipotassium hydrogen phosphate, calcium chloride 0.1 part.
[0020] The preparation method steps of multi-strain fermentation to produce bacteriocin feed rich in taurine are as follows:
[0021] Step 1. Preparation of beef extract-peptone medium: accurately weigh 3g of beef extract, 5g of peptone, and 10g of sodium chloride into a beaker, add 1000mL of water and stir evenly with a glass rod, then adjust the pH to 7.0;
[0022] Preparation of potato culture medium: peel potatoes, cut into pieces, add water and boil for 30 minutes, wherein potatoes: water = 1:4, then filter with gauze, then add 20g of glucose, stir well and make up water to 1000mL;
[0023] Preparation of Cha's Medium: Accurately weigh 2g of sodium nitrate, 1g of dipotassium hydrogen phosphat...
Embodiment 2
[0029] Embodiment 2: The test adopts a single factor test design, selects grass carp with an initial body weight of about 10g, and randomly divides it into 4 treatments, each treatment has 3 repetitions, and each repetition has 20 fish. The experiment was carried out at the base, and the size of the indoor circulating temperature-controlled glass aquarium was 120cm×80cm×80cm, and the test was carried out for a period of 60 days. Feed 3 times a day, respectively at 8:00, 12:00, and 16:00. On the basis of the amount of fish meal in the control group formula being 51%, 24%, 34% and 44% of bacteriocin feed were used to replace 16%, 23% and 31% of the fish meal in the formula, and the effect of bacteriocin feed instead of fish meal was studied. Effects on growth performance of grass carp (determined indicators include survival rate, weight gain rate, feed factor and specific growth rate).
[0030] from image 3 It can be seen that compared with the control group, the breeding effec...
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Abstract
The invention discloses a preparation method of taurine-enriched bacterial peptide feed produced by multi-strain fermentation of sesame meal, and relates to the technical field of agricultural and animal husbandry livestock . The preparation method comprises the following steps: preparing a beef extract peptone culture medium, preparing a potato culture medium, preparing a czapeks culture medium, and activating and culturing strains: evenly mixing raw materials for carrying out fermentation production of the taurine-enriched bacterial peptide feed to obtain a mixture; sterilizing the mixture by adopting steam at high temperature of 110 to 130 DEG C; cooling to form a solid fermentation material; inoculating seed bacterial suspension prepared in the step 2 into the sterilized solid fermentation material according to a volume rate of 4 to 8 percent, and culturing for 65 to 70 hours to obtain the taurine-enriched bacterial peptide feed. The fermented product contains a variety of polypeptides, rich nutrition is provided, and full absorption of the nutrition is promoted. The sesame meal as a fermentation substrate is wide in source and low-cost; therefore, fish meal can be completely or partially replaced with the taurine-enriched bacterial peptide, so that the cost of the feed is reduced.
Description
Technical field: [0001] The invention relates to a preparation method for producing taurine-rich bacteriocin feedstuff by fermenting sesame meal with multiple strains, and belongs to the technical field of agriculture and animal husbandry. Background technique: [0002] The competition in the feed manufacturing industry is fierce, and the price of feed remains high. The shortage of conventional protein raw materials such as soybean meal and fish meal has led to rising prices. The cost of feed has increased sharply, and the income of breeding has continued to decrease. How to reduce the cost of feed has become a widely concerned issue in the market. The most commonly used method to reduce costs is to use more economical sesame meal, cotton meal, etc. to replace part of the traditional high-quality soybean meal and fish meal. [0003] Sesame meal, cotton meal and other plant-based proteins lack taurine, an important substance, compared with fish meal. For livestock, poultry an...
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