An eight immortal flower leaf extract and its drug composition, preparation method and application

A technology of leaf extract and hydrangea, applied in the field of preparation, hydrangea leaf extract and pharmaceutical compositions thereof, can solve problems such as the production process blank of total phenolic acid extract of hydrangea, and achieve stable and controllable quality and economical process. , the effect of clear treatment effect

Active Publication Date: 2020-07-28
SICHUAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report that hydrangea macrophylla leaf or its total phenolic acid extract has anti-thyroid cancer, and the production process of hydrangea macrophylla total phenolic acid extract is also blank in the literature at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An eight immortal flower leaf extract and its drug composition, preparation method and application
  • An eight immortal flower leaf extract and its drug composition, preparation method and application
  • An eight immortal flower leaf extract and its drug composition, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of total phenolic acid extract of hydrangea flower leaves (1)

[0034] The dried medicinal materials of 2kg of pulverized hydrangea leaves were added in 60% ethanol for 12 hours in a ratio of 1:5 by weight of the medicinal materials: ethanol solution, and then heated and refluxed for 3 times, each extraction time was 2h, 2h, 2h. Combine the three extracts, filter, recover the solvent, and concentrate to a relative density of 1.00-1.20. The concentrated liquid was extracted three times with petroleum ether and n-butanol respectively, and extracted according to the ratio of concentrated liquid: solvent weight ratio of 1:1. The three n-butanol extracts were combined and concentrated to obtain 67.8 g of the n-butanol extract. The n-butanol extract was dissolved in 1.5L of distilled water, filtered, and the filtrate was adsorbed on a 2kg AB-8 type macroporous resin column, and the adsorption was repeated three times. First, use 3 times the column volume...

Embodiment 2

[0035] Example 2 Preparation of total phenolic acid extract of hydrangea flower leaves (2)

[0036] 2kg of the dried medicinal materials of the pulverized hydrangea leaves were added with 70% ethanol at a weight ratio of 1:10, soaked in 70% ethanol for 12 hours, and then heated and refluxed for 3 times, each extraction time was 2h, 2h , 2h. Combine the three extracts, filter, recover the solvent, and concentrate to a relative density of 1.00-1.20. The concentrated liquid was extracted three times with petroleum ether and n-butanol respectively, and the concentrated liquid: solvent weight ratio was 1:1. The three n-butanol extracts were combined and concentrated to obtain 85.3 g of the n-butanol extract. The n-butanol extract was dissolved in 2.8L of distilled water, filtered, and the filtrate was adsorbed on a 4kg HPD300 type macroporous resin column, and the adsorption was repeated three times. For the first time, use 4 times the column volume of distilled water and 5 times t...

Embodiment 3

[0037] Example 3 Preparation of total phenolic acid extract of hydrangea flower leaves (3)

[0038] The dried medicinal materials of 2kg of pulverized hydrangea leaves were added with 90% ethanol at a weight ratio of 1:20 in the ratio of medicinal materials: ethanol solution for 24 hours, and then heated and refluxed for 3 times, each extraction time was 3h, 3h , 3h. Combine the three extracts, filter, recover the solvent, and concentrate to a relative density of 1.00-1.20. The concentrated liquid was extracted three times with petroleum ether and n-butanol respectively, and the concentrated liquid: solvent weight ratio was 1:1. The three n-butanol extracts were combined and concentrated to obtain 167 g of n-butanol extract. The n-butanol extract was dissolved in 2.0L of distilled water, filtered, and the filtrate was adsorbed on a 3kg D101 macroporous resin column, and the adsorption was repeated three times. For the first time, use 6 times the column volume of distilled wate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of traditional Chinese medicine and natural medicine pharmacy, and relates to hydrangea leaf extract and its pharmaceutical composition, preparation method and application. The purpose of the present invention is to provide the application of the total phenolic acid extract of hydrangea leaves in the preparation of medicine for treating thyroid cancer. The preparation method of the hydrangea leaf extract of the present invention is as follows: (1) take the hydrangea leaf, pulverize, add a solvent to soak and extract; (2) filter, and concentrate the filtrate for later use; (3) extract the concentrated solution to obtain an extract, extract Add water and filter to obtain the filtrate; (4) adsorb the filtrate to a macroporous resin column, successively use distilled water, 10-20% ethanol aqueous solution, 20-30% ethanol for elution, and 30-40% ethanol for elution, and collect 30-40 % ethanol eluate, concentrated and dried, crushed, and sieved to obtain Hydrangea leaf extract. The total phenolic acid content in the hydrangea leaf total phenolic acid extract is as high as 40%, and the quality is stable and controllable. The extract has a clear therapeutic effect on thyroid cancer through in vivo and in vitro anti-thyroid cancer pharmacological tests.

Description

Technical field [0001] The invention belongs to the field of traditional Chinese medicine and natural medicine pharmacy, and specifically relates to a hydrangea leaf extract and a pharmaceutical composition thereof, a preparation method and an application. Background technique [0002] Hydrangea macrophylla (Hydrangea macrophylla), also known as hydrangea, hydrangea, pink flower, is a perennial herb of the genus Hydrangea in the Saxifraga family. It is native to the south of the middle and lower reaches of the Yangtze River in Mainland China and Honshu, Japan. It has a long history of cultivation in my country and is distributed throughout the north and south of my country. "The Dictionary of Chinese Medicine" records: "Bitterness, slight pungent, cold, and small poison." Its compound cures malaria and cures kidney cystic wind. In recent years, Japanese scholars have conducted many studies on it, and its active ingredients have biological activities such as anti-ulcer and anti-a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P35/00A61K127/00
CPCA61K36/185A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 吴文爽朱精强苏安平龚艳萍杨建洪
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap