Tannin compounds isolated from buckwheat pollen, and applications thereof
A technology of buckwheat pollen and buckwheat bee pollen, which is applied in cosmetics, organic chemistry, drug combination, etc., can solve problems such as ignorance, and achieve the effect of inhibiting prostatitis
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Embodiment 1
[0021] After the buckwheat bee pollen is pulverized, add an ethanol solution with a volume fraction of 80%, the ratio of the buckwheat bee pollen to the ethanol solution is 1:10 (kg / L), stir and extract at 45°C for 3 hours, filter and take the supernatant, This was repeated three times, and the obtained supernatants were combined and concentrated under reduced pressure to obtain the alcoholic extract of buckwheat bee pollen. Extract the buckwheat bee pollen ethanol extract with 2 times the volume of ethyl acetate, extract twice, combine the ethyl acetate extracts, concentrate, and obtain the ethyl acetate extract. Put the ethyl acetate extract on a macroporous resin column, the macroporous resin is AB-8, and carry out gradient elution with deionized water, 30%, 50%, and 95% ethanol solutions with volume fractions of 20mL, 40mL, and 30mL respectively , collect the eluting part with a volume fraction of 50% ethanol solution, concentrate it under reduced pressure and load it on t...
Embodiment 2
[0022] Embodiment 2 Compound A, B structural identification
[0023] from compound A 1 H-NMR (DMSO-d6, 500MHz) spectrum shows that there are five groups of proton signals at δ7.08, δ7.05, δ6.92, δ6.87, δ6.82 (2H, s), and each group contains 2 H , indicating that the compound has a symmetrical structure. There is a H at δ6.39, J=8.0, it is speculated that this H is the signal of the anomeric proton on the sugar, and the signal of other protons on the sugar is at 5.98~δ4.31. from compound A 13 C-NMR (DMSO-d6, 125MHz) spectrum shows that δ91.7, δ72.2, δ72.0, δ70.6, δ67.8, δ61.5 are six carbon signals of sugars on the glucopyranose group . There are five groups of carbon signals at δ165.5~δ164.0, δ145.7~δ145.4, δ139.7~δ138.8, δ118.9~δ117.4, δ109.1~δ108.8, and each group contains 5 carbon. From the spectrum of 135dept, only δ109.1~δ108.8 can be seen, which are CH signals, and the rest are quaternary carbons. According to the above results and referring to relevant literature...
Embodiment 3
[0026] Embodiment 3 is to the inhibitory action of tyrosinase activity
[0027] Table 1 sample addition table
[0028]
[0029] According to Table 1, add a phosphate buffer solution with a pH of 6.8, a sample solution, a tyrosine solution with a concentration of 0.5 g / mL, and an arbutin control solution with a concentration of 1 mg / mL into the test tube in sequence, and place them in a water bath at 35°C for 10 minutes. Then add tyrosinase solution, incubate at 35°C for 30min, quickly transfer to a cuvette, and measure the absorbance value at 475nm. The sample is adjusted to zero with blank control 1, the negative control is adjusted to zero with blank control 2, and the positive control is adjusted to zero with blank control 3. The inhibition rate is calculated as follows
[0030]
[0031] In the formula: A 1 is the absorbance value of the sample; A 2 Absorbance value of negative control; A 3 is the absorbance value of the positive control.
[0032] Table 2 Inhibit...
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