Preparation method and application of a kind of brominated hydrocarbon anaerobic degradation bacterial agent

A technology for degrading bacteria and bacterial agents, applied in the field of biological treatment of groundwater pollution, can solve the problems of high cost, achieve the effects of no secondary pollution, little impact on the surrounding environment, and strong operability

Active Publication Date: 2020-03-24
INST OF SOIL SCI CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical methods include in-situ chemical reduction, in-situ chemical oxidation, etc., and the cost is relatively high

Method used

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  • Preparation method and application of a kind of brominated hydrocarbon anaerobic degradation bacterial agent
  • Preparation method and application of a kind of brominated hydrocarbon anaerobic degradation bacterial agent
  • Preparation method and application of a kind of brominated hydrocarbon anaerobic degradation bacterial agent

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Embodiment 1

[0016] Preparation method of brominated hydrocarbon anaerobic degradation bacterial agent

[0017] Specific steps are as follows:

[0018] (1) Preparation and subpackage of anaerobic medium: Prepare inorganic salt medium and subpackage into 100mL anaerobic bottles.

[0019] (2) Deoxygenate the anaerobic bottle: Use a nitrogen blowing instrument to blow nitrogen into the anaerobic bottle for 30 minutes, measure the dissolved oxygen content in the anaerobic bottle with a dissolved oxygen meter, and seal the anaerobic bottle after the oxygen removal is completed.

[0020] (3) Sterilization: Put the anaerobic bottle into an autoclave and sterilize at 121° C. for 30 minutes.

[0021] (4) Inoculation: After the sterilization is completed, put the anaerobic bottle containing the medium into the anaerobic incubator for anaerobic operation, add 10 mL of anaerobic sludge, seal the anaerobic bottle with a gland clamp and take it out.

[0022] (5) Add standard solution: Add EDB standard...

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Abstract

The invention discloses a preparation method and application of a bromo-hydrocarbon anaerobic degrading bacterial agent. The preparation method comprises the following steps: adding an inorganic salt medium in an anaerobic bottle, removing dissolved oxygen in the medium by utilizing a nitrogen evaporator, after high-temperature and high-pressure sterilization of the medium, adding anaerobic sludge from urban sewage treatment plants in an anaerobic cultural incubator, sealing the anaerobic bottle with a cover presser, injecting an EDB standard liquid in the anaerobic bottle, placing the anaerobic bottle in a constant-temperature shaking incubator for anaerobic culture under the condition with the temperature being 30 DEG C, carrying out transferring after microorganisms reproduce to a logarithmic growth phase, basically removing solid particulates in the anaerobic bottle after transferring for 4-5 times, and effectively enabling anaerobic degrading bacteria to be enriched. The anaerobic degrading bacterial agent provided by the invention can efficiently degrade EDB and is suitable for bioremediation of groundwater contaminated by bromo-hydrocarbon.

Description

technical field [0001] The invention relates to the field of controlling groundwater pollution by biological methods, in particular to a preparation method and application of a brominated hydrocarbon anaerobic degradation bacterial agent. Background technique [0002] Brominated hydrocarbons, such as brominated flame retardants, brominated alkanes, etc., as a class of typical persistent organic pollutants, mostly have "three-pollution" effects and have great potential harm to human health, which has aroused widespread concern . Therefore, research on the remediation of brominated hydrocarbon pollutants in the groundwater environment has important practical significance for protecting groundwater resources, reducing harm to human health and the ecological environment, and promoting sustainable economic and social development. [0003] At present, groundwater pollution remediation technologies are mainly divided into physical methods, chemical methods, biological remediation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/36C12N1/20C02F3/34C02F3/28C02F101/36C02F103/06C12R1/145C12R1/01
CPCC02F3/28C02F3/34C02F2101/36C02F2103/06C12N1/20C12N1/36
Inventor 宋昕杨淼焱王晴唐诗月
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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