New applications of melatonin in inhibition of plant oomycetes diseases, and novel plant oomycetes bactericide

A bactericide and melatonin technology, applied in the field of melatonin, can solve the problem of less melatonin, and achieve the effects of reducing the amount of use, improving the efficacy of pesticides, and weakening the ability to infect

Inactive Publication Date: 2017-11-07
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on melatonin in microorganisms, especially by directly a

Method used

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  • New applications of melatonin in inhibition of plant oomycetes diseases, and novel plant oomycetes bactericide
  • New applications of melatonin in inhibition of plant oomycetes diseases, and novel plant oomycetes bactericide
  • New applications of melatonin in inhibition of plant oomycetes diseases, and novel plant oomycetes bactericide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 prepares medium for experiment

[0026] 1. Rye solid medium (RSA): ① Soak 60g rye grains in 300ml distilled water, 4°C for 36 hours to avoid deterioration; ② Pour out the soaking water and store in the refrigerator, add 100ml of distilled water was mashed for 1-2min, and the suspension was placed in a water bath at 50°C for 3h; ④ Filter the suspension with 3-4 layers of gauze and save the filtrate; Add 20g of sucrose, then add water to 1000ml; ⑥Finally add 12g of agar, autoclave at 121°C for 20min.

[0027] 2. Potato Dextrose Agar Medium (PDA): Weigh 44.3g of commercial PDA medium powder, dissolve it in 1L of distilled water, and sterilize under high pressure at 121°C for 20min.

Embodiment 2

[0028] Embodiment 2 bacteria living experiment

[0029] 1. Treatment of potato leaves, potato cubes and tobacco leaves with melatonin alone

[0030] Potato leaves, potato cubes and tobacco leaves were treated with melatonin, and then the lesion area of ​​the host plants was counted. Potato leaves and potato cubes with a volume of 2cm×3cm×3mm were soaked with different concentrations of melatonin (1mM, 3mM, 6mM, 8mM and 10mM) for 3-5 minutes, and then 7mm infestation bacteria were inoculated into potato leaves and On the potato cubes, place them at 18° C. under the conditions of a photoperiod of 12h:12h, for 5 days. Soak the tobacco leaves with different concentrations of melatonin (1mM, 3mM, 4mM and 6mM) for 3-5 minutes, then inoculate the tobacco leaves with a 7mm block of tobacco black shank bacteria, and place them at 27°C with a photoperiod of 12h:12h Next, 3 days.

[0031] According to the statistical lesion area (such as figure 1 Shown) can be found, with melatonin t...

Embodiment 3

[0036] Embodiment 3 germ in vitro experiment

[0037] 1. Treatment of potato late blight and tobacco black shank with melatonin

[0038] Configure rye solid medium, then add melatonin to the medium, and the final concentration of added melatonin is (1mM, 1.5mM, 2mM, 3mM and 5mM). Take 7mm potato infestans bacterial block and place it in the middle of the culture medium, seal it and place it in the dark at 18°C ​​for 14 days; configure PDA medium, then add melatonin to the medium, the final concentration of melatonin added is (2mM, 4mM, 6mM, 8mM and 9mM). Take tobacco black shank bacterial block of 7 mm and place it in the middle of the medium, seal it and place it at 27°C in the dark for 18 days.

[0039] Observe the growth status of the two pathogens (such as Figure 5 , 6 Shown) It can be found that with the increase of melatonin concentration, the inhibition of bacterial growth is more and more obvious. Treat Potato infestans with melatonin, the drug concentration of I...

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Abstract

The invention discloses new applications of melatonin in inhibition of plant oomycetes diseases, and also discloses a bactericide containing melatonin. The invention discloses for the first time that the melatonin can effectively prevent plant oomycetes diseases, thus providing a new concept for the prevention of oomycetes diseases; melatonin plus fluopicolide or melatonin plus ethylicin combination plays a synergistic effect, the combination use can greatly enhance the pesticide effect, reduce the usage amount of pesticide, and is beneficial to green control and to realize the sustainable development of agricultural ecological civilization.

Description

technical field [0001] The invention relates to a new application of melatonin, in particular to a new application of melatonin for inhibiting plant oomycete diseases and a novel plant oomycete fungicide. Background technique [0002] Oomycetes belong to the kingdom Chromomycota, and because of their morphological characteristics and living habits are similar to fungi, they are usually called fungi; but in terms of evolutionary status, oomycetes are far from fungi, and have closer kinship with brown algae and diatoms. Thus forming a unique group of diploid microorganisms. Oomycetes can infect many plants in agricultural and forestry production, causing a variety of devastating diseases. [0003] Potato late blight, caused by Phytophthora infestans, is a devastating oomycete disease that causes potato stem and leaf death and tuber rot. The disease is regarded as the number one crop disease in the world. Phytophthora infestans belongs to the genus Phytophthora oomycetes. Th...

Claims

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Application Information

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IPC IPC(8): A01N43/38A01N41/00A01N43/40A01N47/12A01P3/00A01G13/00
CPCA01G13/00A01N41/00A01N43/38A01N43/40A01N47/12A01N2300/00
Inventor 任茂智张蜀敏张建奎刘森
Owner CHONGQING UNIV
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