Planting method for transgenic paphiopedilum emersonii

Abstract

The invention discloses a planting method for transgenic paphiopedilum emersonii. The planting method comprises the following steps: seedling dip-dyeing, seedling dark culture, seedling light culture, shed framing, seedling transplanting, seedling bed management and control on plant diseases and insect pests. According to the planting method disclosed by the invention, LBA4404 agrobacterium tumefaciens liquid is dip-dyed with paphiopedilum emersonii seedlings to change gene sequence thereof, the paphiopedilum emersonii seedling which is dip-dyed and cultured for 30 days is soaked for 3 hours in a 37-DEG C constant-temperature box with an X-Glue staining solution, is taken out and subjected to discoloring treatment through liquor, so that a deep blue compound which is visible by naked eyes can be generated, and therefore, gene conversion of the paphiopedilum emersonii is proved to occur. The paphiopedilum emersonii seedling subjected to transgenosis is taken as a culture object to perform shed greenhouse culture, so that the cultured paphiopedilum emersonii is good in growth condition, flowering phase is advanced by nearly one month, flower odor is thicker, a lesion rate is lower, flowers are greater; and moreover, flower opening time is obviously prolonged. The planting method is worthy of being popularized.

Description

technical field [0001] The invention relates to the technical field of flower planting, in particular to a method for planting transgenic Paupideopsis alba Background technique [0002] Paphiopedilum is a ground or semi-epiphytic plant. Ye Jisheng, several to many pieces, blade band-shaped, leathery. Scapes grow from the leaves, bracts are non-leaf; the top of the ovary is often narrowed into a beak shape; the flowers are large and showy, with various colors; the middle calyx is erect, the pollen is powdery or sticky, and the staminodes are flat; the stigma Hypertrophic, recurved, with mastoids on the stigma, and the fruit is a capsule. It blooms from April to May. The orchid is more elegant, the color is more solemn, and the color has irregular spots or stripes. The petals are thicker and the bloom period is longer. It is an ornamental flower. The habitat of P. alba in the crevices of steep rock walls is bathed in black lime soil, the soil is scarce, and the roots are...

AI Technical Summary

Problems solved by technology

[0005] At present, in the flower market, most of the Paphiopedilum orchids are cultivated in greenhouses, but the growth habits of the orchids are unconventional, and the artificially cultivated Paphiopedilum is far inferior to the wild ones in terms of flowering period, flower color, flower shape, and aroma. Orchid, and it is vulnerable to pests and diseases, as well as bacterial and fungal infections during the cultivation process, and the growth of Paphiopedilum is relatively slow, prone to disease, and the flowering period is delayed