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A kind of day-neutral strawberry tissue culture rapid propagation medium and tissue culture rapid propagation method

A technology of tissue culture, rapid propagation and medium, applied in tissue culture, horticultural methods, botanical equipment and methods, etc., can solve problems such as susceptible to viruses, aggravation of seedling diseases and insect pests, and different medium formulations, methods, and procedures. , to achieve the effect of fast reproduction rate and high reproduction rate

Active Publication Date: 2019-01-22
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The commonly used propagation methods of strawberries are stolon propagation and branch propagation. Their propagation speed is slow, and they are susceptible to viruses, which will lead to aggravated pests and diseases of seedlings and reduced yields.
In order to improve the reproduction speed and cultivate virus-free seedlings, one-season strawberry uses stolon tip tissue culture for rapid propagation, but it is difficult for day-neutral strawberries to produce stolons, so they can only be propagated by ramets. Base formula and its method, program are also different, there is not yet the report of the rapid propagation method of day-neutral strawberry

Method used

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  • A kind of day-neutral strawberry tissue culture rapid propagation medium and tissue culture rapid propagation method
  • A kind of day-neutral strawberry tissue culture rapid propagation medium and tissue culture rapid propagation method
  • A kind of day-neutral strawberry tissue culture rapid propagation medium and tissue culture rapid propagation method

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Experimental program
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Effect test

Embodiment 1

[0058] 1) Select day-neutral strawberry leaves that are robust, bright green in color, and free from diseases and insect pests, soak them in alkaline detergent for 30 minutes, then rinse them under running water for 1 hour, rinse them repeatedly with distilled water for 4 to 5 times, and place them on a sterile operating table ;

[0059] 2) Soak in 75% ethanol for 10-20 seconds on the sterile operating table, rinse with sterile water for 4-6 times, then disinfect the surface with 0.8%-3% sodium hypochlorite for 10-15 minutes, rinse with sterile water for 4-6 Put it into a sterile petri dish and cut it, take the leaves and cut them obliquely at an angle of 45°C along the veins, cut into pieces with a length of 10-20 mm and a width of 7-10 mm, blot the water dry on sterile filter paper, and set aside;

[0060] 3) Dark culture the cut leaves on callus induction medium B5 solid medium + BA3.4mg / L + IBA 0.3mg / L + sucrose concentration of 3%, pH 5.8-6.0, and insert buds after 2 week...

Embodiment 2

[0067] 1) Selection and pretreatment of explants: Select the cryptobuds at the base of the crown stems of daily neutral strawberries that grow robustly, are bright green in color, and are free from diseases and insect pests, soak them in alkaline detergent for 30 minutes, rinse them under running water for 1 hour, and rinse them with distilled water. Repeatedly rinse 4 to 5 times and place on a sterile operating table;

[0068] 2) Soak in 70% ethanol for 10-20 seconds on the sterile operating table, rinse with sterile water for 4-6 times, then disinfect the surface with 0.8%-3% sodium hypochlorite for 10-15 minutes, rinse with sterile water for 4-6 Second-rate;

[0069] 3) Peel off the tips of the cryptobuds in the crown diameter after sterilization, about 0.1-0.3 cm, insert them into the bud induction medium, and place them in the light cultivation room for static cultivation, and wait for the young buds to grow to 0.3-0.5 cm for proliferation and culture; The bud induction ...

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Abstract

The invention relates to a day-neutral strawberry tissue culture and rapid propagation culture medium, and a tissue culture and rapid propagation method, and belongs to the technical field of strawberry tissue culture and rapid propagation. The propagation method provided by the invention comprises the following steps: 1) selecting explants; 2) sterilizing the explants; 3) performing shoot induced culture; 4) performing shoot elongation culture; 5) performing proliferation culture; 6) performing rooting culture; and 7) hardening seedlings and transplanting. According to the propagation method provided by the invention, latent buds of day-neutral strawberry leaves or crownshafts serve as the explants, the formula of the culture medium with the growth hormone concentration reduced continuously is adopted, and large-scale day-neutral strawberry seedlings can be obtained within half a year.

Description

technical field [0001] The invention relates to the technical field of tissue culture rapid propagation of strawberry, in particular to a daily neutral strawberry tissue culture rapid propagation medium and a tissue culture rapid propagation method. Background technique [0002] Strawberry is the first choice for high-efficiency agriculture, with short production cycle, quick results and high income. At present, the main strawberries planted in production are one-season strawberries, which are planted in autumn and produce fruit in winter and spring. Greenhouse insulation is used to promote cultivation or open field overwintering cultivation mode, so that the strawberry fruiting period is from mid-November to May of the next year, but from June to October There is still no fresh strawberry fruit to eat; day-neutral strawberry is a four-season strawberry. Its breeding and planting successfully solved the problem of no supply of fresh strawberry fruit in summer and autumn, whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00C12N5/02A01H4/00
CPCA01H4/001A01H4/008
Inventor 王静
Owner JIANGSU ACAD OF AGRI SCI
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