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Preparation method for three-dimensional cell scaffold based on collagen sponge-nanocellulose

A nanocellulose and collagen sponge technology, applied in the field of biomaterials and tissue engineering, can solve the problems of easy degradation, poor mechanical strength, limited application, etc., and achieve the effects of reliable results, easy operation and strong repeatability

Inactive Publication Date: 2017-11-17
武汉枫霖科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, due to the poor mechanical strength and easy degradation, the application in medicine and tissue engineering is greatly limited. Therefore, it is especially important to improve the scaffold performance of collagen sponge to achieve wide application.

Method used

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  • Preparation method for three-dimensional cell scaffold based on collagen sponge-nanocellulose
  • Preparation method for three-dimensional cell scaffold based on collagen sponge-nanocellulose
  • Preparation method for three-dimensional cell scaffold based on collagen sponge-nanocellulose

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Embodiment Construction

[0022] The implementation of the present invention will be illustrated by specific specific examples below, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0023] Such as figure 1 Shown is the morphological figure of the collagen sponge-nanocellulose cell scaffold of the present invention;

[0024] The preparation method of three-dimensional cell scaffold based on collagen sponge-nanocellulose, through the preparation of collagen sponge, the preparation of nanocellulose, the preparation and cultivation steps of collagen sponge-nanocellulose, complete the three-dimensional cell based on collagen sponge-nanocellulose The preparation method of support; Concrete steps are as follows:

[0025] (1) Preparation of collagen sponge: degrease, separate beef tendons, trotters, fish skin, cartilage, tendon or skin tissue, digest them completely in acetic acid solution of pepsin, ...

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Abstract

The invention discloses a preparation method for a three-dimensional cell scaffold based on collagen sponge-nanocellulose. The preparation method is characterized in that the preparation method for the three-dimensional cell scaffold based on the collagen sponge-nanocellulose is completed through the steps of preparation of collagen sponge, preparation of nanocellulose and preparation and cultivation of the collagen sponge-nanocellulose. The preparation method for the three-dimensional cell scaffold based on the collagen sponge-nanocellulose is reliable in result, easy and convenient to operate and high in repeatability, three-dimensional growth and proliferation of cells can be effectively promoted for a long time, meanwhile, a certain effect is achieved on formation of various human body three-dimensional micro-tissues, especially on formation of the liver cancer tissue function, and thus beneficial reference can be provided for the scientific research of liver tissue engineering and clinical treatment of liver cancers.

Description

technical field [0001] The invention belongs to the technical field of biomaterials and tissue engineering, and specifically relates to a [0002] Preparation method of collagen sponge-nanocellulose three-dimensional cell scaffold. Background technique [0003] Collagen is a structural protein that exists in the body in the form of collagen fibers. Collagen extracted from fresh porcine Achilles tendon, bovine Achilles tendon, etc. can maintain a triple helical structure, can re-form fibers under simulated physiological conditions, can obviously activate cell growth, and has good biological activity and tissue repair function. However, due to the poor mechanical strength and easy degradation, the application in medicine and tissue engineering is greatly limited. Therefore, it is especially important to improve the scaffold performance of collagen sponge to achieve wide application. [0004] TEMPO oxidized nanocellulose dispersion (TOCN) has the characteristics of large spec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/56
CPCA61L27/26A61L27/56A61L2400/12A61L2430/28C08L1/00C08L89/00
Inventor 崔晓峰高桂芳
Owner 武汉枫霖科技有限公司
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