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SNP Molecular Markers Related to Upland Cotton Score and Its Application

A molecular marker, cotton coat technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc.

Active Publication Date: 2020-11-20
HEBEI AGRICULTURAL UNIV. +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The level of lint production directly affects the purchase price of seed cotton and the income of cotton farmers. Cotton content is a quantitative trait controlled by multiple genes, which is easily affected by the environment. In addition, there is a negative correlation between fiber quality and yield. The use of conventional breeding methods to improve cotton fiber quality progress slow

Method used

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  • SNP Molecular Markers Related to Upland Cotton Score and Its Application
  • SNP Molecular Markers Related to Upland Cotton Score and Its Application
  • SNP Molecular Markers Related to Upland Cotton Score and Its Application

Examples

Experimental program
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Embodiment 1

[0058] 1. Determination of cotton content: 419 cotton core germplasm resources field experiments were carried out in Cangzhou of Hebei, Anyang of Henan, Jingzhou of Hubei, Yancheng of Jiangsu, Dunhuang of Gansu and Alar of Xinjiang for two consecutive years in 2014 and 2015 respectively. . Residential Area 6m 2 , 1 row per plot, row spacing 0.8m, plant spacing 0.3m, observation track 0.8m, isolation track 1.2m, 20 plants per repetition. The experiment was set to repeat 3 times, and the normal field cultivation management. During the peak period of cotton bolling, harvest 1-2 bolls of normal bolls on the first and second fruit nodes in the middle of each normal cotton plant, a total of 20 bolls. After ginning, calculate the clothing percentage of each variety (the ratio of seed cotton per unit weight to ginned lint, expressed as a percentage in the calculation).

[0059] 2. Detection of SNP: Take the young leaves of cotton plants, and use the plant genome extraction kit to e...

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Abstract

The invention relates to molecular markers, in particular to SNP molecular markers related to the lint percentage of upland cotton and application thereof. The molecular markers are shown as one of SEQ ID NO. 1- SEQ ID NO. 1049; a mutation occurs at the 500 bp site, so that polymorphism of the lint percentage occurs. The SNP molecular markers related to the lint percentage of the upland cotton can be used for early prediction and screening of the cotton lint percentage trait, and can also be used for molecular marker-assisted selection of the cotton lint percentage. The SNP molecular markers are directly presented as the form of DNA, can be detected at various tissue and various developmental stages of the cotton, are not restricted by the season and the environment, and the problem whether or not the SNP molecular markers are expressed does not exist.

Description

technical field [0001] The present invention relates to molecular markers, in particular to SNP molecular markers related to upland cotton. Background technique [0002] Cotton is an important fiber and oil crop in the world, and cotton production has a huge impact on the global economy. Cloth content is one of the main components of cotton lint yield. The level of lint production directly affects the purchase price of seed cotton and the income of cotton farmers. Cotton content is a quantitative trait controlled by multiple genes, which is easily affected by the environment. In addition, there is a negative correlation between fiber quality and yield. The use of conventional breeding methods to improve cotton fiber quality progress slow. It is of great significance to use molecular marker technology to excavate the control genes of cotton traits to increase cotton yield, improve the income of cotton farmers, and promote the development of cotton industry. [0003] SNP (s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6895C40B40/06
CPCC12Q1/6895C12Q2600/13C12Q2600/156C40B40/06
Inventor 马峙英王省芬杜雄明李志坤张桂寅何守朴吴立强孙君灵张艳杨君吴金华贾银华潘兆娥王国宁柯会锋
Owner HEBEI AGRICULTURAL UNIV.