Preparation method and application of hypochloric acid-responsive fluorescence sensing material

A technology of fluorescent sensing and hypochlorous acid, which is applied in the field of chemical fluorescent sensing materials, can solve problems such as unsatisfactory detection limit and low fluorescence quantum yield, and achieve sensitive and selective recognition performance, large optical performance, and detection low limit effect

Inactive Publication Date: 2017-11-21
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these fluorescent sensing materials already have some disadvantages in terms of low fluorescence quantum

Method used

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  • Preparation method and application of hypochloric acid-responsive fluorescence sensing material
  • Preparation method and application of hypochloric acid-responsive fluorescence sensing material
  • Preparation method and application of hypochloric acid-responsive fluorescence sensing material

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1: Preparation of hypochlorous acid-responsive fluorescent chemical sensing material

[0032] Weigh 0.81 g of 6-carboxycoumarin and 0.92 g of thiosemicarbazide into a round bottom flask, add 5 mL of anhydrous methanol to dissolve, and heat at 80°C for 4 hours. Filter while hot, recrystallize the filtrate, filter with suction, and wash three times with 5 mL of anhydrous methanol to obtain a white solid.

Example Embodiment

[0033] Example 2: Preparation of hypochlorous acid-responsive fluorescent chemical sensing material

[0034] Weigh 1.19g of 6-carboxycoumarin and 1.84g of thiosemicarbazide into a round bottom flask, add 15mL of anhydrous methanol to dissolve, and heat at 100°C for 6h. Filter while hot, recrystallize the filtrate, filter with suction, and wash three times with 15 mL of anhydrous methanol to obtain a white solid.

Example Embodiment

[0035] Example 3: Preparation of hypochlorous acid-responsive fluorescent chemical sensing material

[0036] Weigh 1 g of 6-carboxycoumarin and 1.38 g of thiosemicarbazide into a round bottom flask, add 10 mL of anhydrous methanol to dissolve, and heat at 90° C. for 5 hours. Filter while hot, recrystallize the filtrate, filter with suction, and wash with 10 mL of anhydrous methanol three times to obtain a white solid.

[0037] Such as figure 1 Shown is a schematic diagram of the synthesis process of hypochlorous acid-responsive fluorescent sensing materials.

[0038] Such as figure 2 Shown is the infrared spectrum of the fluorescent sensing material. FT-IR(KBr,cm -1 ): 3369,3264,3178,1745,1684,1645,1618,1568,1532,1421,1374,1317,1286,1227,1207,1165,999,802,770,647. The functional groups of fluorescent sensing materials can be determined by infrared spectroscopy.

[0039] Such as image 3 with Figure 4 Shown are the fluorescent sensing materials 1 H NMR and 13 C NMR chart. 1 H NMR(D...

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Abstract

The invention relates to a preparation method and application of a hypochloric acid-responsive fluorescence sensing material, belonging to the technical field of chemiluminescence sensing materials. The hypochloric acid-responsive fluorescence sensing material provided by the invention is a chemiluminescence sensing material prepared through an electrophilic substitution reaction with coumarin-6-carboxylic acid and thiosemicarbazide as basic raw materials and absolute methanol as a solvent. The fluorescence sensing material prepared in the invention has good water-solubility and biocompatibility, is rapid in response, and can realize sensitive and selective recognition of OCl<->; the changes of fluorescence signals are visible to naked eyes under the illumination of an ultraviolet lamp; interference caused by other common negative ions and active oxygen substances is small; and the fluorescence sensing material successfully realizes imaging of target ions in biological cells.

Description

technical field [0001] The invention belongs to the technical field of chemical fluorescent sensing materials, relates to a preparation method and application of a fluorescent sensing material, and mainly studies a preparation method and application of a hypochlorous acid-responsive fluorescent sensing material. Background technique [0002] As a kind of reactive oxygen species, hypochlorous acid plays an important role in many life sciences. Hypochlorous acid is produced by the myeloperoxidase-catalyzed reaction between hydrogen peroxide and chloride ions, which protects living systems from microorganisms and pathogens. And uncontrolled hypochlorous acid from phagocytes can cause various diseases such as cardiovascular disease, inflammation and cancer. In addition, hypochlorous acid is also widely used as a safe disinfectant in drinking water and swimming pools, and as a bleaching agent in industrial applications. Strictly controlling the amount of hypochlorous acid in ou...

Claims

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Application Information

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IPC IPC(8): C07D311/18C09K11/06G01N21/64
Inventor 李文轩王蕾智文静宋丹瞿敏侃戴震倪良
Owner JIANGSU UNIV
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