Pharmaceutical composition and extract for reversing tumor multidrug resistance

A multi-drug resistance, extract technology, applied in drug combinations, anti-tumor drugs, pharmaceutical formulations, etc., can solve problems such as reducing the effect of chemical drug treatment, and achieve excellent effect of reversing tumor drug resistance.

Inactive Publication Date: 2017-11-24
徐钉锈
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Multidrug resistance means that when tumor cells are exposed to a certain chemotherapeutic drug for a long time during chemotherapy, they will not only develop resistance to this drug, but also to other chemotherapeutic drugs with different structures and functions, thus greatly reducing the risk of cancer. Effects of Chemotherapy

Method used

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  • Pharmaceutical composition and extract for reversing tumor multidrug resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Preparation of the extract of toad venom that reverses the multidrug resistance of tumors

[0022] (1) Add 100g of toad venom to 1L of 95% ethanol aqueous solution, soak at 25°C for 3 days, and filter to obtain the ethanol extract;

[0023] (2) Concentrate the ethanol extract until it has no alcohol smell, and then use a non-polar macroporous resin column to enrich the active ingredients of bufotoxin;

[0024] The specific method for enriching the effective components of bufatoxins in the non-polar macroporous resin column is as follows: after loading the ethanol extract on the non-polar macroporous resin column (D101 macroporous resin column), first eluting with water to remove Miscellaneous, and then use 80% ethanol to elute the non-polar macroporous resin column; collect the ethanol eluted parts, concentrate and dry to obtain the toad venom extract.

[0025] After testing, the active ingredients of toad toxins in the toad venom extract prepared in this exa...

Embodiment 2

[0029] Example 2 Preparation of the extract of toad venom that reverses the multidrug resistance of tumors

[0030] (1) Add 100g of toad venom to 1L of 95% ethanol aqueous solution, soak at 25°C for 3 days, and filter to obtain the ethanol extract;

[0031] (2) Concentrate the ethanol extract until it has no alcohol smell, and then use a non-polar macroporous resin column to enrich the active ingredients of bufotoxin;

[0032] The specific method for enriching the effective components of bufatoxins in the non-polar macroporous resin column is as follows: after loading the ethanol extract on the non-polar macroporous resin column (D101 macroporous resin column), first eluting with water to remove Miscellaneous, and then use 60% ethanol to elute the non-polar macroporous resin column; collect the ethanol eluted parts, concentrate and dry to obtain the toad venom extract.

[0033] After testing, the active ingredients of toad toxins in the toad venom extract prepared in this exa...

Embodiment 3

[0034] Example 3 Preparation of the extract of toad venom that reverses the multidrug resistance of tumors

[0035] (1) Add 100g of toad venom to 1L of 95% ethanol aqueous solution, soak at 25°C for 3 days, and filter to obtain the ethanol extract;

[0036] (2) Concentrate the ethanol extract until it has no alcohol smell, and then use a non-polar macroporous resin column to enrich the active ingredients of bufotoxin;

[0037] The specific method for enriching the effective components of bufatoxins in the non-polar macroporous resin column is as follows: after loading the ethanol extract on the non-polar macroporous resin column (D101 macroporous resin column), first eluting with water to remove Miscellaneous, and then use 40% ethanol to elute the non-polar macroporous resin column; collect the ethanol eluted parts, concentrate and dry to obtain the toad venom extract.

[0038] After testing, the toad toxin active ingredients in the Bufa toad extract prepared in this example ...

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Abstract

The invention relates to the technical field of pharmaceutical chemistry and in particular discloses a pharmaceutical composition and a extract for reversing tumor multidrug resistance. The pharmaceutical composition for reversing the tumor multidrug resistance is characterized by being prepared from the following components in parts by weight: 30 to 60 parts of arenobufagin, 8 to 20 parts of hellebrigenin, 10 to 25 parts of telocinobufagin, 25 to 50 parts of bufotaline, 20 to 40 parts of cinobufotalin, 25 to 50 parts of bufalin, 30 to 60 parts of cinobufagin and 15 to 30 parts of resibufogenin. Cinobufagin venom toad extract takes the pharmaceutical composition as an effective component. The reverse multiple of the pharmaceutical composition on the tumor drug resistance is 30 to 70 times; the reverse multiple of the extract on the tumor drug resistance is 10 to 20 times.

Description

technical field [0001] The invention relates to the technical field of medicinal chemistry, in particular to a pharmaceutical composition and an extract for reversing multi-drug resistance of tumors. Background technique [0002] Tumor (tumour) refers to the neogrowth formed by the proliferation of local histiocytes under the action of various tumorigenic factors, because most of these neogrowths are space-occupying block-like protrusions, also known as neoplasms (neoplasm) . According to the cell characteristics of new organisms and the degree of harm to the body, tumors are divided into two categories: benign tumors and malignant tumors, and cancer is the general term for malignant tumors. [0003] Currently, chemotherapy is the main method of cancer treatment, and cancer patients who receive long-term chemotherapy will develop multidrug resistance (MDR) during treatment. Multidrug resistance means that when tumor cells are exposed to a certain chemotherapeutic drug for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/585A61P35/00
CPCA61K31/585A61K2300/00
Inventor 徐钉锈
Owner 徐钉锈
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