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Drugs, applications and methods for controlling the transmission of geminiviruses for inhibiting the transmission of geminiviruses carried by whitefly

A technology of geminivirus and Bemisia tabaci, applied in the field of suppressing the spread of Geminiviruses carried by Bemisia tabaci, and controlling the spread of geminiviruses, can solve problems such as not being very effective

Active Publication Date: 2020-04-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the problem that the method of controlling the spread of geminivirus in the field by killing Bemisia tabaci is not very effective in the prior art, and provides a new drug for inhibiting the spread of Geminivirus carried by Bemisia tabaci and a method for controlling the spread of Geminivirus method

Method used

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  • Drugs, applications and methods for controlling the transmission of geminiviruses for inhibiting the transmission of geminiviruses carried by whitefly
  • Drugs, applications and methods for controlling the transmission of geminiviruses for inhibiting the transmission of geminiviruses carried by whitefly
  • Drugs, applications and methods for controlling the transmission of geminiviruses for inhibiting the transmission of geminiviruses carried by whitefly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Effect of BrefA on the ability of Bemisia tabaci to acquire tomato yellow leaf curl virus.

[0028] (1) Dissolving BrefA in ethanol to form a mother solution with a concentration of 5 mg / ml;

[0029] (2) Dilute the BrefA mother liquor in 30% sucrose solution at a dilution ratio of 1:1000 to prepare a BrefA inhibitor solution;

[0030] (3) Feeding Bemisia tabaci through double-layer Parafilm membrane method. One end of the double-pass glass prepared in advance is covered with two layers of Parafilm films, and the prepared BrefA inhibitor solution or the control solution containing equal proportion of ethanol is sandwiched between the two layers of Parafilm films. Then a certain amount of Bemisia tabaci is introduced into the double-way pipe from the other end and sealed with gauze.

[0031] (4) After 24 hours of feeding, the Bemisia tabaci fed with inhibitor and without inhibitor sucrose solution were transferred to tomatoes infected with tomato yellow leaf curl virus ...

Embodiment 2

[0038] Effects of BrefA on geminivirus content in the hemolymph of whitefly whitefly.

[0039] (1) Dissolving BrefA in ethanol to form a mother solution with a concentration of 5 mg / ml;

[0040] (2) Dilute the BrefA mother liquor in 30% sucrose solution at a dilution ratio of 1:1000 to prepare a BrefA inhibitor solution;

[0041] (3) Feeding Bemisia tabaci through double-layer Parafilm membrane method. One end of the double-pass glass prepared in advance is covered with two layers of Parafilm films, and the prepared BrefA inhibitor solution or the control solution containing equal proportion of ethanol is sandwiched between the two layers of Parafilm films. Then a certain amount of Bemisia tabaci is introduced into the double-way pipe from the other end and sealed with gauze.

[0042] (4) After 24 hours of feeding, the Bemisia tabaci fed with sucrose solutions containing inhibitors and without inhibitors were transferred to tomato plants infected with tomato yellow leaf curl...

Embodiment 3

[0048] The effect of BrefA on the transmission of geminiviruses by whiteflies.

[0049] (1) Dissolving BrefA in ethanol to form a mother solution with a concentration of 5 mg / ml;

[0050] (2) Dilute the BrefA mother liquor in 30% sucrose solution at a dilution ratio of 1:1000 to prepare a BrefA inhibitor solution;

[0051] (3) Feeding Bemisia tabaci through double-layer Parafilm membrane method. One end of the double-pass glass prepared in advance is covered with two layers of Parafilm films, and the prepared BrefA inhibitor solution or the control solution containing equal proportion of ethanol is sandwiched between the two layers of Parafilm films. Then a certain amount of Bemisia tabaci is introduced into the double-way pipe from the other end and sealed with gauze.

[0052] (4) After 24 hours of feeding, the Bemisia tabaci fed with inhibitor and without inhibitor sucrose solutions were transferred to tomatoes infected with tomato yellow leaf curl virus (TYLCV) for 12 hou...

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Abstract

The invention discloses a medicine for inhibiting geminivirus carried and transmitted by bemisia tabaci, application and a method for controlling geminivirus transmission. The medicine comprises one or a combination of an early internal transport inhibitor and a cytoskeleton inhibitor. According to study, early internal transport proteins and cytoskeleton proteins of bemisia tabaci and coding genes of the proteins can serve as targets for inhibiting geminivirus carried and transmitted by bemisia tabaci, by virtue of an inhibitor BrefA or after genes of the early internal transport proteins and cytoskeleton proteins are subjected to RNA interference silent expression, the ability of the bemisia tabaci for carrying and transmitting the geminivirus can be effectively inhibited, so that field transmission of the geminivirus can be effectively controlled.

Description

technical field [0001] The invention relates to the technical field of geminivirus prevention and control, in particular to a drug for inhibiting the transmission of geminiviruses carried by bemisia tabaci, its application and a method for controlling the spread of geminiviruses. Background technique [0002] Geminivirus is a kind of plant single-stranded circular DNA virus with twin particle shape. After infecting plants, it can lead to crop yield reduction and cause serious harm to agricultural production worldwide. The spread of such viruses mainly depends on the feeding of vector insects. Traditionally, growers usually control vector insects by applying pesticides and other means to protect plants from viruses. However, due to insect resistance and other reasons, the above conventional methods are difficult to control the spread of geminiviruses, so the development of new alternative strategies has gradually become a research hotspot. [0003] Geminiviridae mainly inclu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/89C12N15/87A01K67/033
CPCA01K67/0335A01K2227/706C12N15/87C12N15/89
Inventor 夏文强王晓伟
Owner ZHEJIANG UNIV
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