A test strip for detecting low-density lipoprotein cholesterol in serum and its preparation method
A technology of low-density lipoprotein and test strips, which is applied in the field of test strips for detecting low-density lipoprotein cholesterol in serum and its preparation, and can solve the problems of high price, complicated operation, and cumbersome preparation methods, etc.
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Embodiment 1
[0045] (1) Preparation of total cholesterol (TC) detection reagent: according to cholesterol lipase (CHER) 5KU / L, cholesterol oxidase (CHOD) 40KU / L, MgSO 4 280mM, horseradish peroxidase 150KU / L, 4-aminoantipyrine 18 mM, N-ethyl-N-(2-hydroxy-3-propanesulfonyl) m-toluidine (TOOS) 45 mM concentration , using phosphoric acid (PBS) as a buffer, add all the above reagents into a 15mL EP tube, and mix well.
[0046] ) In the reagent obtained in the first step, add 0.14g Tween-20 (Tween-20) to 1mL reagent, and then add poloxamer F124 with a number average molecular weight of 8400 at a concentration of 1.8g / L, and mix well.
[0047] ) Cut the nitrocellulose membrane with a pore size of 0.4um into strips about 0.5cm wide, put them into the solution prepared in the previous step, and soak for 1.5 hours. Then take it out, put it into an electric blast drying box, and dry it for 60 minutes at 45 degrees Celsius. The completely dried membrane strip is the LDL-c dry chemical reaction shee...
Embodiment 2
[0052] (1) Preparation of total cholesterol (TC) detection reagent: according to cholesterol lipase (CHER) 15KU / L, cholesterol oxidase (CHOD) 60KU / L, MgSO 4 320mM, horseradish peroxidase 250KU / L, 4-aminoantipyrine 22 mM, N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt (TOPS) 55 mM Concentration, with phosphoric acid (PBS) as the buffer solution, add all the above reagents into a 15mL EP tube, and mix well.
[0053] ) To the reagent obtained in the first step, add 0.18 g Tween-20 (Tween-20) to 1 mL reagent, and then add poloxamer F88 with a number average molecular weight of 8400 at a concentration of 2.2 g / L, and mix well.
[0054] ) Cut the nitrocellulose membrane with a pore size of 0.4um into strips about 0.5cm wide, put them into the solution prepared in the previous step, and soak for 1.5 hours. Then take it out, put it into an electric blast drying box, and dry it for 60 minutes at 45 degrees Celsius. The completely dried membrane strip is the LDL-c dry chemical...
Embodiment 3
[0059] (1) Preparation of total cholesterol (TC) detection reagent: according to cholesterol lipase (CHER) 10KU / L, cholesterol oxidase (CHOD) 50KU / L, MgCl 2 300mM, horseradish peroxidase 200KU / L, 4-aminoantipyrine 20 mM, nitro blue tetrazolium chloride (NBT) 50 mM concentration, with phosphoric acid (PBS) as buffer, the above reagents Add all to a 15mL EP tube and mix well.
[0060] ) In the reagent obtained in the first step, add 0.16g NP-10 to 1mL reagent, and then add poloxamer F88 with a number average molecular weight of 6000 at a concentration of 2g / L, and mix well.
[0061] ) Cut the nitrocellulose membrane with a pore size of 0.4um into strips about 0.5cm wide, put them into the solution prepared in the previous step, and soak for 1.5 hours. Then take it out, put it into an electric blast drying box, and dry it for 60 minutes at 45 degrees Celsius. The completely dried membrane strip is the LDL-c dry chemical reaction sheet.
[0062] ) Use a 2mm thick glass fiber f...
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