Nanometer selenium hydrosol having antitumor activity, preparation method, preservation method and applications thereof

A technology of anti-tumor activity and nano-selenium, which is applied in the direction of anti-tumor drugs, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems that the biological activity of nano-selenium cannot be effectively improved, and avoid adverse effects and avoid aggregation The effect of simple precipitation and preparation steps

Active Publication Date: 2017-12-01
THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST +2
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  • Abstract
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  • Claims
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Problems solved by technology

[0006] The object of the present invention is to provide a nano-selenium hydrosol with anti-tumor activity and a preparation method thereof for the problem that existing nano-selenium preparation methods cannot effectively improve the biological activity of nano-selenium through modifiers or regulators

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  • Nanometer selenium hydrosol having antitumor activity, preparation method, preservation method and applications thereof
  • Nanometer selenium hydrosol having antitumor activity, preparation method, preservation method and applications thereof
  • Nanometer selenium hydrosol having antitumor activity, preparation method, preservation method and applications thereof

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[0040] Correspondingly, on the basis of providing the nano-selenium hydrosol with anti-tumor activity, the present invention further provides a preparation method of the nano-selenium hydrosol with anti-tumor activity. In a specific embodiment, the preparation method of the nano-selenium hydrosol with anti-tumor activity at least includes the following steps:

[0041] Step S01. Adding a solution containing selenium ions and / or selenium ions to the polysaccharide protein aqueous solution of versicolor versicolor;

[0042] Step S02. Add a reducing agent solution dropwise to the mixed solution obtained in step S01, and oscillate;

[0043] Step S03. The product obtained from the reduction reaction in step S02 is subjected to constant volume and dialysis treatment.

[0044] In any embodiment, the versicolor polysaccharide protein in the versicolor polysaccharide protein aqueous solution is versicolor versicolor water-soluble polysaccharide protein.

[0045] As preferably, in the em...

Embodiment 1

[0064] (1) At 25°C and 101.325kPa, 0mL, 0.5mL, 1.0mL, 3.0mL, 6.0mL of Versicolor versicolor water-soluble polysaccharide protein with a mass concentration of 2.5g / L were added to 5 mL of double-distilled water containing 10.0mL respectively. In a 25mL volumetric flask;

[0065] (2) Add 1.0 mL of sodium selenite solution with a concentration of 0.025 mol / L to the above-mentioned 25 mL volumetric flask respectively, shake it up gently, and make it fully mixed to obtain a mixed solution;

[0066] (3) To the mixed solution in (2), add 1.0 mL of vitamin C solution with a concentration of 0.1 mol / L dropwise, shake gently while adding, add water to make up to 25.0 mL, and let stand , until the red color no longer deepens, and then dialyzed (molecular weight cut-off 8000) for 24 hours, the product can be obtained. The content of selenium was measured by nitration ICP method. It can be seen that the concentration of nano-selenium in the obtained product is basically about 1.0-1.5mmol / ...

Embodiment 2

[0080] (1) At 20°C and 101.325kPa, take 3.0mL of Versicolor versicolor water-soluble polysaccharide protein aqueous solution with a mass concentration of 2.5g / L and add them to four 25mL volumetric flasks filled with 10mL of double-distilled water;

[0081] (2) Add 0.1mL, 0.5mL, 1.0mL, 2.0mL of sodium selenite solutions with a concentration of 0.025mol / L to the above-mentioned 25mL volumetric flask, shake gently, and make it fully mixed to obtain a mixed solution;

[0082] (3) Add dropwise 0.1mL, 0.5mL, 1.0mL, 2.0mL of vitamin C (English abbreviation: Vc) solutions with a concentration of 0.1mol / L to the mixed solution in (2), shake gently while adding After the addition is complete, add water to make up to 25mL, let it stand still until the red color no longer deepens, and then dialyze (molecular weight cut-off 8000) for 24 hours to obtain the product. The selenium content is measured by the nitration ICP method, and it can be known that the nano-selenium concentration in the o...

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Abstract

The present invention relates to the technical field of nanometer selenium hydrosols, particularly to a nanometer selenium hydrosol having antitumor activity, a preparation method, a preservation method and applications thereof, wherein the hydrosol at least contains nanometer selenium with a concentration of 0.5-5.0 mmol / L, and Coriolus versicolor polysaccharide protein with a concentration of 100.0-600.0 mg / L. The preparation method at least comprises: S01, adding a solution containing selenium ions and / or selenous ions to a Coriolus versicolor polysaccharide protein aqueous solution; S02, adding a reducing agent solution to the mixed solution obtained in the step S01 in a dropwise manner, and shaking; and S03, carrying out volume metering on the product obtained after the reducing reaction, and carrying out dialysis treatment. According to the present invention, the hydrosol is stable at a temperature of 2-10 DEG C; and the Coriolus versicolor polysaccharide protein has the hydrophilic hydroxyl and the hydrophilic amino, and the two groups can enhance the affinity between the nanometer selenium and the cancer cells, and can improve the uptake on the nanometer selenium by tumor cells so as to achieve the whole treatment effect of medication dosage reducing, treatment effect improving and less toxic-side effect, such that the effective scheme is provided for the combination chemotherapy of cancers in the clinic.

Description

technical field [0001] The invention relates to the technical field of nano-selenium hydrosol, in particular to a nano-selenium hydrosol with anti-tumor activity and its preparation, preservation method and application. Background technique [0002] Selenium is one of the fifteen essential trace elements in the human body. It has important biological activities in the human body such as anti-tumor, anti-oxidation, anti-aging, improving immunity, protecting and repairing cells, and antagonizing and reducing harmful heavy metals. Selenium is closely related to the occurrence and development of as many as 40 diseases, including: AIDS (AIDS), liver cancer, Keshan disease, Kashin-Beck disease, cardiovascular and cerebrovascular diseases. [0003] People with low selenium or selenium deficiency can improve the body's immunity and maintain the normal functions of important organs such as the heart, liver, lungs, and stomach by supplementing an appropriate amount of selenium. At the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07A61K38/02A61K33/04A61K9/10A61K47/42A61P35/00
CPCA61K9/10A61K33/04A61K36/07A61K38/02A61K47/42
Inventor 黄家兴陈填烽
Owner THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
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