Method for degrading bisphenol-C by utilizing white-rot fungus crude enzyme

Abstract

The invention discloses a method for degrading bisphenol-C by utilizing a white-rot fungus crude enzyme. White-rot fungus lyophilized powder is inoculated to a potato dextrose agar slant culture-medium, and activated strain spores are cultured out for stand-by application; in the inoculating process, the stand-by activated strain spores are washed down by using sterile water and inoculated to a liquid potato dextrose culture-medium, shake cultivation is conducted, and the culture-medium inoculated with white-rot fungi is obtained; the culture-medium inoculated with the white-rot fungi is transferred to a centrifuge cup, and supernatant and mycelia are obtained through centrifugation; the lower-layer mycelia are removed, the supernatant is subjected to centrifugation operation for one to two times, the supernatant after being subjected to centrifugation operation is filtered by a 0.45 [mu]m filter membrane, and a white-rot fungus extracellular crude enzyme is obtained; and a MnSO4 solution, H2O2, a tartaric acid-sodium tartrate buffer solution, the white-rot fungus extracellular crude enzyme and a bisphenol-C-methanol solution are mixed, extracellular enzyme reaction system mixed liquor is built, and after 2-5 days, degradation of the bisphenol-C can be achieved. According to the method for degrading the bisphenol-S by utilizing the white-rot fungus crude enzyme, the cost is low, operation is simple and convenient, and the treatment capacity is large.

Description

technical field [0001] The invention belongs to the technical field of environmental restoration, and in particular relates to a method for degrading bisphenol-C by utilizing crude enzyme liquid of microorganism-white rot fungus. Background technique [0002] Bisphenol C (bisphenol C, BPC), as one of the typical representatives of environmental endocrine disrupting chemicals (EDCs), has an effect similar to estrogen, and a small amount of ingestion in the body can destroy the endocrine system of the human body and cause infertility , teratogenic, etc., its biological toxicity and endocrine disrupting activity are equivalent to bisphenol A, and they have broad application prospects as emerging chemical materials. After bisphenol C compounds enter the human body, they can dissolve in fat and then accumulate in the body, making it difficult to excrete them from the body. Studies have shown that bisphenol C is found in human serum. Therefore, it is urgent to find a way to degra...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for degrading bisphenol C by using white rot fungus crude enzyme solution to solve the problems of large workload, high cost, low processing capacity and cumbersome steps in the process of BPC degradation in the prior art.