Method for degrading bisphenol-S by utilizing white-rot fungus crude enzyme

A technology of white rot fungus and crude enzyme solution, which is applied in the field of environmental restoration, can solve the problems of low processing capacity, cumbersome steps, and heavy workload, and achieve the effects of high degradation rate, efficient catalytic ability, and low cost

Inactive Publication Date: 2017-12-01
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for degrading bisphenol S by using white rot fungus crude enzyme solution to solve the problems of heavy workload, high cost, low processing capacity and cumbersome steps in the process of BPS degradation in the prior art.

Method used

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Embodiment

[0014] In order to make the technical means, goals and effects of the present invention more clear and easy to understand, the present invention will be further elaborated below in conjunction with specific embodiments, and the listed examples are only intended to illustrate the present invention and not limit the present invention.

[0015] (1) Cultivation of white rot fungi

[0016] Inoculate the lyophilized powder of white rot fungus on the potato dextrose agar slant medium, culture at 37°C for 5 days, store it at 4°C under sterile conditions, and use it as activated spores for later use. Rinse down the spores of the activated strains, inoculate them into liquid potato glucose medium, place them in a shaking culture at 37°C and 130 r / min for 5 days, and control the OD value to 1.5 during inoculation to obtain cultures inoculated with white rot fungi base.

[0017] (2) Preparation of extracellular crude enzyme solution of white rot fungi

[0018] Move the culture medium in...

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Abstract

The invention discloses a method for degrading bisphenol-S by utilizing a white-rot fungus crude enzyme. White-rot fungus lyophilized powder is inoculated to a potato dextrose agar slant culture-medium, and activated strain spores are cultured out for stand-by application; in the inoculating process, the stand-by activated strain spores are washed down by using sterile water and inoculated to a liquid potato dextrose culture-medium, shake cultivation is conducted, and the culture-medium inoculated with white-rot fungi is obtained; the culture-medium inoculated with the white-rot fungi is transferred to a centrifuge cup, and supernatant and mycelia are obtained through centrifugation; the lower-layer mycelia are removed, the supernatant is subjected to centrifugation operation for one to two times, the supernatant after being subjected to centrifugation operation is filtered by a 0.45 [mu]m filter membrane, and a white-rot fungus extracellular crude enzyme is obtained; and a MnSO4 solution, H2O2, a tartaric acid-sodium tartrate buffer solution, the white-rot fungus extracellular crude enzyme and a bisphenol-S-methanol solution are mixed, extracellular enzyme reaction system mixed liquor is built, and after 2-5 days, degradation of the bisphenol-S can be achieved. According to the method for degrading the bisphenol-S by utilizing the white-rot fungus crude enzyme, the cost is low, operation is simple and convenient, and the treatment capacity is large.

Description

technical field [0001] The invention belongs to the technical field of environmental restoration, and in particular relates to a method for degrading bisphenol-S by utilizing crude enzyme liquid of microorganism-white rot fungus. Background technique [0002] Bisphenol S (bisphenol S, BPS), the chemical name is 4,4-dihydroxydiphenyl sulfone, white needle crystal, is an important intermediate of pesticides, dyes and auxiliaries. There are many kinds of products synthesized from bisphenol S, including engineering plastic polyarylsulfone, bisphenol S epoxy resin, new H-class insulating material polyethersulfone, etc., which can be used to manufacture laminated products, molding compounds, powder coatings Flame retardants, etc. Studies have shown that long-term exposure of BPS to the environment may have adverse effects on the central nervous system, immune system, reproductive system and thyroid hormones of humans and animals. Wayne, a UCLA researcher, has shown that BPA accel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D3/02A62D101/28
CPCA62D3/02A62D2101/28
Inventor 刘红艳马钰邢贺张泽雄王秀丽
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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