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A nano-microbubble mixture providing biological light source and preparation method thereof

A hybrid, nanotechnology, applied in the fields of nanotechnology, nanotechnology, nano-optics, etc. for materials and surface science, can solve problems such as hindering further application of photodynamic therapy, inability to guarantee activity, tissue damage, etc., and achieve easy penetration. Tissue and cell membrane, ensure biological activity and stability, uniform particle size effect

Active Publication Date: 2020-12-29
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, enzymes or substrates are easily degraded quickly after entering the body, and the activity cannot be guaranteed
[0005] In addition, the targeting of photodynamic therapy in liver cancer is also a problem that needs to be solved urgently. Since the existing photodynamic therapy rarely involves research on deep tissues such as the liver, it can be applied to other superficial tissues based on photodynamic therapy. The sensitivity mainly depends on the passive targeting effect of the retention effect of the photosensitizer on the tumor tissue, as well as the targeted irradiation of the manipulated light source.
This passive targeting with low specificity can easily cause damage to normal tissues, which hinders the further application of photodynamic therapy.

Method used

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  • A nano-microbubble mixture providing biological light source and preparation method thereof
  • A nano-microbubble mixture providing biological light source and preparation method thereof

Examples

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Embodiment 1

[0046] see figure 1 and figure 2As shown, the present invention provides a nano-microbubble mixture of a biological light source, which includes: folic acid receptor-targeted enzyme-loaded nano-bubbles, the enzyme-loaded nano-bubbles are composed of a first core template and a first shell, and the first The core template is a CdSe / ZnS quantum dot-coelenterazine 8 complex, and the first shell is a folic acid cross-linked polyethylene glycol polylactic-glycolic acid polymer; folic acid receptor-targeted substrate-loaded nanomicrobubbles , the substrate-loaded nano-microbubble is composed of a second core template and a second shell, the second core template is CdSe / ZnS quantum dot-coelenterazine complex and / or coelenterazine, the second The shell is folic acid cross-linked polyethylene glycol poly(lactic-co-glycolic acid) polymer. Wherein, the CdSe / ZnS quantum dot-coelenterazine 8 complex is formed by covalent coupling of CdSe / ZnS quantum dots and coelenterazine 8, and the Cd...

Embodiment 1

[0053] The preparation method of the nano-microbubble mixture providing a biological light source provided by the present invention has the advantages of stable encapsulation efficiency, uniform particle size, high yield, The advantages of simple synthetic processing methods, the mixture is easy to store, which includes:

[0054] The step of preparing the enzyme-loaded nano-microbubbles;

[0055] The step of preparing the substrate-loaded nano microbubbles.

Embodiment 2

[0057] The difference between this embodiment and the preparation method Example 1 is that in the step of preparing the enzyme-loaded nano-microbubbles, the present invention provides the preparation steps of the enzyme-loaded nano-microbubbles as follows:

[0058] S1: preparing a folic acid cross-linked polyethylene glycol poly(lactic-co-glycolic acid) polymer, and making it form the first shell;

[0059] S2: preparing the CdSe / ZnS quantum dot-coelenterazine 8 complex and making it form the first core template;

[0060] S3: loading the first core template into the first shell to complete the preparation of the enzyme-loaded nano-microbubbles.

[0061] At the same time, the present invention also provides the preparation steps of the substrate-loaded nano-microbubbles, the preparation method of which is the same as the preparation of the enzyme-loaded nano-microbubbles, only the first core template needs to be replaced with the second core As for the template, the first shell...

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Abstract

The invention discloses a nano-microbubble mixture for providing a biological light source, which comprises: folate receptor-targeted enzyme-loaded nano-bubbles and folate receptor-targeted substrate-loaded nano-bubbles, and the enzyme-loaded nano-bubbles are composed of The first core template and the first shell consist of a CdSe / ZnS quantum dot-coelenterazine luciferase 8 complex, and the first shell is a folic acid cross-linked polyethylene glycol polylactic-glycolic acid polymer; The substrate nano-microbubble is composed of a second core template and a second shell, the second core template is CdSe / ZnS quantum dot-coelenterazine complex and / or coelenterazine, and the second shell is folic acid cross-linked polyethylene Diol poly(lactic-co-glycolic acid) polymer. The invention also provides a preparation method of the nano-microbubble mixture. The nano-microbubble mixture provided by the invention has the advantages of targeted release and delayed degradation, and maintains the activity of enzymes and substrates in vivo, and the nano-microbubble mixture can be used as a light source in photodynamic therapy.

Description

technical field [0001] The present invention relates to the field of pharmaceutical active protective agents and the field of synthesis of targeted drug carriers, in particular to a nano-microbubble mixture for providing biological light sources and a preparation method thereof. Background technique [0002] Photodynamic therapy (Photodynamic Therapy, PDT) is a new method to treat tumors after surgery, chemotherapy and radiotherapy. Good therapeutic effects have been achieved in superficial tissue tumors such as skin cancer and breast cancer. At present, photodynamic therapy mainly uses a laser emitter as an external light source to excite a photosensitizer (Photosensitizer, PS) that is injected intravenously and distributed in the tumor tissue, thereby triggering a photodynamic reaction (Photodaynamic Reaction, PDR) to generate a cytotoxic agent - reactive oxygen species (reactive oxygen species). Oxygen Species, ROS), leading to necrosis and / or apoptosis of tumor cells, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51
CPCB82Y20/00B82Y30/00B82Y40/00C09K11/025C09K11/883
Inventor 王立宇周星李加欢宋祥铭程翔郑启昌
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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