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Micro-fluidic chip and method for preparing cell droplets

A microfluidic chip and microfluidic technology, applied in the field of biomedical application instruments, can solve the problems of inability to accurately extract single-cell droplets and inability to accurately separate single-cell single-cell droplets, etc., to improve the preparation accuracy and preparation Efficiency, Reduction of Reagent Contamination, Effect of Avoiding Contamination

Active Publication Date: 2017-12-15
SHENZHEN INST OF ADVANCED TECH
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Problems solved by technology

[0003] However, existing technologies cannot precisely separate single cells to obtain single-cell droplets, nor can they accurately extract single-cell droplets for subsequent reactions

Method used

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  • Micro-fluidic chip and method for preparing cell droplets
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  • Micro-fluidic chip and method for preparing cell droplets

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Embodiment Construction

[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Apparently, the described embodiments are some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0033] An embodiment of the present invention provides a microfluidic chip, including n microfluidic units, where n is a positive integer. For example, n=1, 2, 3... When n≥2, n microfluidic units are connected sequentially, and each microfluidic unit has the same structure. Among them, each microfluidic unit can produce a cell droplet. The following embodiments of the present invention are described by taking n=1 and n=2 as examples. It should be understood that th...

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Abstract

The invention provides a micro-fluidic chip and a method for preparing cell droplets by using the micro-fluidic chip. The micro-fluidic chip comprises a micro-fluidic unit; a first flow channel, a second flow channel, a third flow channel and a fourth flow channel are arranged in the micro-fluidic unit; the first flow channel, the second flow channel and the third flow channel are sequentially connected; both the first flow channel and the third flow channel are crossed with the second flow channel; two pneumatic micro valves are arranged on the second flow channel; a position where the second flow channel is connected with the first flow channel and the third flow channel is positioned between the pneumatic micro valves; a pneumatic micro valve is arranged on each of the first flow channel and the third flow channel; the opposite two ends of the fourth flow channel are communicated with the second flow channel; the internal diameter of the fourth flow channel is set to be capable of capturing at least one cell and be less than the inner diameter of the second flow channel. With the adoption of the scheme of the invention, the cell droplets can be accurately prepared and extracted.

Description

technical field [0001] The invention relates to the field of biomedical application instruments, in particular to a microfluidic chip and a method for preparing cell droplets. Background technique [0002] With the continuous development of genomics and proteomics research, more and more researchers are no longer limited to genetic research at the multi-cellular level, but are focusing on the more specific level of a single cell. Therefore, the rapid isolation of single cells and the acquisition of single cell droplets have become the key technologies for single cell research and analysis. [0003] However, the existing technology cannot accurately separate single cells to obtain single-cell droplets, nor can it accurately extract single-cell droplets for subsequent reactions. Contents of the invention [0004] In view of this, the present invention provides a microfluidic chip and a method for preparing cell droplets using the microfluidic chip. [0005] A microfluidic ...

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Application Information

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IPC IPC(8): C12M1/00
CPCC12M23/16
Inventor 张宝月陈艳
Owner SHENZHEN INST OF ADVANCED TECH
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