A sodium alginate-chitosan polyion complex hydrogel and its 3D printing preparation method

Abstract

The invention discloses a sodium alginate-chitosan polyionic compound hydrogel and its 3D-printing preparation method. The compound hydrogel is the sodium alginate-chitosan polyionic compound hydrogelof which carboxylate radicals are crosslinked with ammonium ions, the biocompatibility is good, cell loss caused by material degradation in the cell culture process using hydrogel blocks can be effectively improved, and cells cultured on the materials easily obtain required oxygen and nutrient substances. The 3D-printing preparation method includes the following steps of preparation of a sodium alginate-chitosan polyionic compound solution and preparation of the sodium alginate-chitosan polyionic compound hydrogel. The sodium alginate-chitosan polyionic compound hydrogel is prepared by usingthe 3D-printing technology, the outside dimensions are controllable, pore structures which are visible in the macroscopic aspect and interconnected are obtained, combination between hydrogel materialsand tissue is easily achieved, and after material implantation, wear of materials at a tissue loss site is reduced.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a sodium alginate-chitosan polyion composite hydrogel and a 3D printing preparation method thereof. Background technique [0002] Sodium alginate is a polyanionic polysaccharide sodium salt extracted from brown algae. It has good biocompatibility and can interact electrostatically with divalent anions or polyanionic macromolecules to form a hydrogel three-dimensional network structure. Chitosan is a macromolecule obtained by deacetylation of methine molecules. Due to the interaction between amino groups on the molecular chain, chitosan macromolecules form crystals in water, resulting in insoluble chitosan. In the acid solution, the amino groups on the chitosan molecular chain are converted into ammonium ions to form polyanionic macromolecules, which can interact electrostatically with polycations or cationic small molecules to form a three-dimensional cross-linked ne...

AI Technical Summary

Problems solved by technology

Sodium alginate-chitosan polyion complex hydrogel forms a three-dimensional cross-linked network through electrostatic interactions. When used as tissue engineering scaffold materials, the presence of small molecules such as sodium ions and chloride ions in the body will destroy the formation of electrostatic interactions. The three-dimensional cross-linked network leads to the rapid degradation of the hydrogel scaffold material, and the degradation process is gradually degraded from the surface. During the degradation process, the mechanical properties of the hydrogel material decrease rapidly, and it is not conducive to the adhesion and proliferation of cells cultured on the surface of the material.

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