A method for extracting isoquercitrin from bidens pilosa L.

A technology of isoquercitrin and ghost needle grass, applied in the field of extracting isoquercitrin, can solve the problems of low selectivity, oil loss of aromatic compounds, unsatisfactory extraction effect, etc.

Inactive Publication Date: 2018-01-09
桂林纽泰生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the common solvent C0 2 It has the characteristics of non-polarity and small relative molecular mass, which makes the extraction effect unsatisfactory due to its low solubility and low selectivity for some substances. In actual operation, it is often necessary to increase the extraction rate by adding entraining agents. Finally, it is necessary to try to remove the entraining agent in the volatile oil, which will inevitably lead to the complexity of the process and the loss and oxidation of volatile components; in addition, because water is insoluble in CO 2 , so the supercritical C0 2 The extraction process often needs to pre-dry the raw materials, which increases the cost and causes the loss of aromatic compound oil
In addition, when extracting volatile oils from plants, due to supercritical CO 2 It has similar solubility not only to volatile oils but also to low-polarity compounds such as waxes, fatty acids, colored substances and resins in the stratum corneum. These substances will also be extracted during the extraction process, although complex systems can also be used. Pure volatile oil, but the operation is cumbersome and the requirements for equipment are more stringent

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  • A method for extracting isoquercitrin from bidens pilosa L.
  • A method for extracting isoquercitrin from bidens pilosa L.
  • A method for extracting isoquercitrin from bidens pilosa L.

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Embodiment 1

[0024] The method for extracting isoquercitrin from the ghost needle grass, specifically comprises the following steps:

[0025] 1) Clean the ghost needle grass, dry it, crush it with a pulverizer, and pass it through a 20-mesh sieve;

[0026] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 185-200°C, pressure 8-10Mpa, extraction time 0.5h, extract the ghost needle grass, the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature, and the extract is obtained;

[0027] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography throug...

Embodiment 2

[0029] The method for extracting isoquercitrin from the ghost needle grass, specifically comprises the following steps:

[0030] 1) Clean the ghost needle grass, dry it, crush it with a pulverizer, and pass it through a 40-mesh sieve;

[0031] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 185-200°C, pressure 8-10Mpa, extraction time 1.0h, extract the ghost needle grass, the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature, and the extract is obtained;

[0032] 3) Purification: Concentrate the extract obtained in step 2), control the temperature to ≤50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, and perform column chromatography ...

Embodiment 3

[0034] The method for extracting isoquercitrin from the ghost needle grass, specifically comprises the following steps:

[0035]1) Clean the ghost needle grass, dry it, crush it with a pulverizer, and pass through an 80-mesh sieve;

[0036] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 185-200°C, pressure 8-10Mpa, extraction time 1.0h, extract the ghost needle grass, the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature, and the extract is obtained;

[0037] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through D...

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Abstract

A method for extracting isoquercitrin from bidens pilosa L. is disclosed. The method includes 1) a step of cleaning and airing the bidens pilosa L., crushing the bidens pilosa L. by utilizing a crusher, and sieving the crushed bidens pilosa L.; 2) a step of performing subcritical water extraction, namely a step of adding the treated raw material into an extraction kettle, adding hydroxyethyl cellulose the weight of which is 0.001 part of the weight of the raw material into the kettle, injecting deoxygenated deionized water into the kettle, extracting the bidens pilosa L. at 185-200 DEG C under8-10 MPa for 0.5-1.0 h, after the extraction is finished, performing pressure relief, allowing an extraction liquid to flow into a collection tank, and cooling the extraction liquid to room temperature to obtain the extraction liquid; and 3) a step of purification, namely a step of concentrating the extraction liquid obtained in the step 2), performing column chromatography utilizing macroporousresin, performing elution with an ethanol solution having a concentration of 50% v/v to obtain an eluate, concentrating the eluate, and drying a concentration product to obtain a finished product.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting isoquercitrin from P. Background technique [0002] Isoquercitrin, alias: quercetin-3-O-glucoside, isoquercetin, apocynum. Molecular formula: C 21 h 20 o 12 , molecular weight: 464.38. Yellow needle-like crystals (water), melting point 225-227°C, almost insoluble in cold water, slightly soluble in boiling water, soluble in alkali solution to appear dark yellow. Yellow crystal (ethanol), melting point 206-208°C and 232-236°C. Pale yellow needle crystal, melting point 220-222°C (decomposition). Crystallization (hot water), melting point 230°C. Animal experiments have hypotensive effect. Tests such as capillary permeability have shown anti-inflammatory effects. It has a poisonous effect on the larvae of the cotton budworm. It has enzyme-lowering effect and is one of the active ingredients of Tian Jihuang in treating hepatitis. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 谢冬养
Owner 桂林纽泰生物科技有限公司
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