Ramaria botrytoides refined polysaccharide as well as preparation method and application thereof

A technology of coral bacteria and polysaccharides, applied in the field of medicine, to achieve the effect of enhanced release and increased expression

Inactive Publication Date: 2018-01-09
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are almost no research reports on polysaccharides from coral bacteria.

Method used

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  • Ramaria botrytoides refined polysaccharide as well as preparation method and application thereof
  • Ramaria botrytoides refined polysaccharide as well as preparation method and application thereof
  • Ramaria botrytoides refined polysaccharide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Preparation of Coral Fungus Crude Polysaccharides (RBPs) from Coral Fungus:

[0055] (1) After crushing the coral bacteria, pass through a 20-mesh sieve, weigh 100g, heat reflux with distilled water for extraction, wherein the water to material ratio is 30:1 (mass ratio), the reflux extraction temperature is 90℃, the reflux extraction time is 1h, reflux Extraction times 3 times; then combine the extracts, centrifuge at 4000r / min for 10min, take the supernatant liquid, filter 3 times with filter paper, and concentrate the filtrate with a rotary evaporator at 40°C under reduced pressure to 200mL to obtain a polysaccharide solution;

[0056] (2) D354FD resin was soaked in distilled water for 12 hours, and then immersed in 5% by mass hydrochloric acid solution and 5% by mass sodium hydroxide solution respectively for 0.5h, filtered with 200 mesh gauze, and washed with distilled water until it became neutral. Obtain pretreated D354FD resin;

[0057] (3) Mix the polysacch...

Embodiment 2

[0059] Example 2 Preparation of Coral Fungus Crude Polysaccharides (RBPs) from Coral Fungus:

[0060] (1) The coral bacteria are crushed and passed through a 20-mesh sieve, weighed 100g, and then heated and refluxed for extraction with distilled water. The ratio of water to material is 30:1 (mass ratio), the reflux extraction temperature is 90℃, reflux extraction time is 1h, reflux extraction The number of times is 2; then the extracts are combined, centrifuged at 3000r / min for 8min, the supernatant liquid is taken, and the filter paper is filtered twice. The filtrate is concentrated to 200mL under reduced pressure with a rotary evaporator at 50°C to obtain a polysaccharide solution;

[0061] (2) D354FD resin was soaked in distilled water for 24 hours, and then immersed in 5% by mass hydrochloric acid solution and 5% by mass sodium hydroxide solution for 2 hours respectively, filtered with 200 mesh gauze, and washed with distilled water until it became neutral. D354FD resin after p...

Embodiment 3

[0064] Example 3 Preparation of Coral Fungus Crude Polysaccharides (RBPs) from Coral Fungus:

[0065] (1) After crushing the coral bacteria, pass through a 20-mesh sieve, weigh 100g, and heat and reflux with distilled water for extraction. The ratio of water to material is 30:1 (mass ratio), the reflux extraction temperature is 95°C, the reflux extraction time is 2h, reflux Extract 4 times, then combine the extracts, centrifuge at 5000 / min for 12 min, take the supernatant liquid, filter 4 times with filter paper, and concentrate the filtrate to 200 mL under reduced pressure with a rotary evaporator at 60°C to obtain a polysaccharide solution;

[0066] (2) D354FD resin was soaked in distilled water for 18 hours, and then immersed in 5% by mass hydrochloric acid solution and 5% by mass sodium hydroxide solution respectively for 3 hours, filtered with 200 mesh gauze, and washed with distilled water to neutrality. D354FD resin after pretreatment;

[0067] (3) Mix the polysaccharide solu...

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Abstract

The invention belongs to the technical field of medicines and discloses a ramaria botrytoides refined polysaccharide as well as a preparation method and application thereof. The method comprises the following steps: (1) carrying out refluxing extraction on ramaria botrytoides in distilled water and carrying out de-coloring treatment on an extracting solution by utilizing macroporous adsorption resin; then precipitating by utilizing a precipitant; centrifuging and collecting sediment, and drying the sediment to obtain a ramaria botrytoides crude polysaccharide; (2) preparing the ramaria botrytoides crude polysaccharide into a polysaccharide solution; transferring the polysaccharide solution into a pre-treated chromatographic column of DEAE (Diethylaminoethyl)-52 resin; eluting the polysaccharide solution with distilled water and collecting eluting solutions respectively; detecting the eluting solutions and determining the absorbance under the wavelength of 490nm; drawing a curve and combining the eluting solutions in the same absorption peak; concentrating and drying to obtain the refined polysaccharide. The polysaccharide disclosed by the invention has anti-oxidization, anti-tumorand immunological enhancement activity and a low utilization dosage, and also has a relatively low toxic side effect under the same dosage.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to a coral fungus refined polysaccharide and a preparation method and application thereof. Background technique [0002] Polysaccharides are an important class of biopolymer compounds other than proteins and nucleic acids. Modern pharmacological studies have shown that polysaccharides have a variety of physiological activities, including anti-oxidation, anti-tumor, anti-virus, lowering blood lipids, delaying aging, and enhancing immune function. Immune regulation is the most important biological activity function of polysaccharides, and it has always been a hot research topic. The adverse reactions and side effects of immune bacterial polysaccharides and synthetic compounds have attracted widespread attention. Most of the polysaccharides derived from higher plants are non-adverse substances and will not cause major side effects to the body. Therefore, polysaccharides isolat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06A61P35/00A61P37/04
Inventor 陈健董芳高呈琳耿佳欢申超群
Owner SOUTH CHINA UNIV OF TECH
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