Culture medium for selenium-enriched lucid ganoderma

A medium and selenium-enriched technology, applied in the field of preparation of selenium-enriched Ganoderma lucidum medium

Inactive Publication Date: 2018-02-02
JINZHAI LIMIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, introducing selenium into the cul...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method for selenium-enriched ganoderma lucidum medium, comprising the following steps:

[0023] (1) Sodium selenite, isomaltulose, probiotics, glutamic acid, and pure water are mixed to form a fermentation broth, and fermented; the probiotics are lactic acid bacteria, bifidobacteria, and Lactobacillus rhamnosus, respectively passed through MRS medium Cultivate for 18 hours, mix it into a strain liquid according to the volume ratio of 2:1:1, add 50 mL of strain liquid per liter of fermentation liquid, add 20 mg of selenium per liter of fermentation liquid, and add isomalt The amount of ketose added is 50g per liter of fermentation broth, and the amount of glutamic acid added is 5g per liter of fermentation broth; the pH of the fermentation broth is adjusted to 5.0, the temperature is adjusted to 35°C, and fermentation is carried out for 16 hours;

[0024] (2) Centrifuge the fermentation broth, remove bacteria and then sterilize and inactivate to obtain a se...

Embodiment 2

[0028] A preparation method for selenium-enriched ganoderma lucidum medium, comprising the following steps:

[0029] (1) Sodium selenate, isomaltulose, probiotics, D-phenylalanine, and pure water were mixed to form a fermentation broth, and fermented; the probiotics were lactic acid bacteria, bifidobacteria, and Lactobacillus rhamnosus, which were passed MRS respectively The culture medium was cultivated for 24 hours, and mixed into a seed liquid according to a volume ratio of 2:1:1. The amount added was 20 mL of the seed liquid per liter of fermentation liquid, and the amount of sodium selenate was 50 mg of selenium per liter of fermentation liquid. The amount of maltulose added is 15g per liter of fermentation broth, and the amount of glutamic acid added is 15g per liter of fermentation broth; the pH of the fermentation broth is adjusted to 6.5, the temperature is adjusted to 30°C, and fermentation is carried out for 32 hours;

[0030] (2) Centrifuge the fermentation broth, ...

Embodiment 3

[0034] A preparation method for selenium-enriched ganoderma lucidum medium, comprising the following steps:

[0035] (1) Sodium selenite, isomaltulose, probiotics, glutamic acid, and pure water are mixed to form a fermentation broth, and fermented; the probiotics are lactic acid bacteria, bifidobacteria, and Lactobacillus rhamnosus, respectively passed through MRS medium Cultivate for 20 hours, and mix it into a seed liquid according to a volume ratio of 2:1:1. The amount added is 40 mL of the seed liquid per liter of fermentation liquid. The amount of sodium selenite added is 30 mg of selenium per liter of fermentation liquid. The amount of ketose to be added is 30g per liter of fermentation broth, and the amount of glutamic acid to be added is 10g per liter of fermentation broth; the pH of the fermentation broth is adjusted to 6.0, the temperature is adjusted to 33°C, and fermentation is carried out for 24 hours;

[0036] (2) Centrifuge the fermentation broth, remove bacteri...

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PUM

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Abstract

The invention discloses a preparation method of a culture medium for selenium-enriched lucid ganoderma. The preparation method comprises the following steps: (1) mixing selenium salt, sugar, probiotics, amino acid and pure water to form a fermentation liquid, and performing fermentation; (2) sterilizing and inactivating the fermentation liquid to obtain a selenium-rich liquid; and (3) mixing 20-30parts by weight of cottonseed hulls, 10-20 parts by weight of wood flour, 8-15 parts by weight of bran, 1-6 parts by weight of vitamin, 10-15 parts by weight of monopotassium phosphate, 5-10 parts byweight of gypsum, 8-16 parts by weight of malt, 2-8 parts by weight of corn flour, 10-20 parts by weight of corncob and 10-20 parts by weight of the selenium-rich liquid to obtain the culture mediumfor selenium-enriched lucid ganoderma. Inorganic selenium is transformed into organic selenium through probiotics fermentation, and the obtained organic selenium is added into the culture medium for the lucid ganoderma, so that the selenium is enriched in the body during the growth process of the lucid ganoderma, and the selenium-enriched lucid ganoderma with high selenium content is cultivated.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, and in particular relates to a preparation method of a selenium-enriched ganoderma medium. Background technique [0002] Ganoderma lucidum is a kind of edible and medicinal fungus, which has the effects of invigorating qi and calming the nerves, relieving cough and relieving asthma. It is a high-quality raw material used to make health care products. The number of wild Ganoderma lucidum is very small. At present, artificially cultivated Ganoderma lucidum is mainly used. [0003] Selenium is an essential trace element for the human body and has important physiological functions. It can prevent and inhibit tumors, resist aging, maintain the normal structure and function of the cardiovascular system, prevent arteriosclerosis and coronary heart disease, and has anti-oxidation and anti-tumor properties. and enhance immunity. Inorganic selenium (such as selenite and selenate) enters the human ...

Claims

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Application Information

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IPC IPC(8): A01G18/20C05G1/00
CPCC05B7/00C05C11/00C05D3/00C05D9/00C05F5/002C05F11/00C05F11/08C05F11/10
Inventor 吴善良赵学群刘新利吴茂林
Owner JINZHAI LIMIN BIOTECH CO LTD
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