Korean ginseng stem cell extract sunscreen cosmetic and preparation method thereof
An extract and stem cell technology, applied in the field of skin care products, can solve problems such as safety to be improved, sunscreen composition changes, skin prone to oxidative deterioration, etc., to repair sunburned skin, strong antioxidant and anti-aging activities, quality stable effect
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Embodiment 1
[0030] An embodiment of the preparation method of the Korean ginseng stem cell extract sunscreen skin care product of the present invention, the preparation method of the Korean ginseng stem cell extract sunscreen skin care product described in this embodiment is:
[0031] (1) Preparation of Korean ginseng stem cell extract
[0032] 1) Isolation and culture of Korean ginseng cambium stem cells
[0033] After the root tuber of Korean ginseng is sterilized, cut into semicircular slices including the cambium;
[0034] Place the semicircular slices on the WPM medium and culture in the dark at 25°C;
[0035] Two weeks later, the external proliferating bodies with obvious cambium proliferation were taken out, and the cambium stem cells were separated and transferred to the subculture medium for culture, and subcultured once every two weeks to obtain Korean ginseng cambium stem cells;
[0036] 2) Collect the culture medium after culturing the Korean ginseng cambium stem cells, filt...
Embodiment 2
[0045] An embodiment of the preparation method of the Korean ginseng stem cell extract sunscreen skin care product of the present invention, the preparation method of the Korean ginseng stem cell extract sunscreen skin care product described in this embodiment is:
[0046] (1) Preparation of Korean ginseng stem cell extract
[0047]1) Isolation and culture of Korean ginseng cambium stem cells
[0048] After the root tuber of Korean ginseng is sterilized, cut into semicircular slices including the cambium;
[0049] Place the semicircular slices on the WPM medium and culture in the dark at 25°C;
[0050] Two weeks later, the external proliferating bodies with obvious cambium proliferation were taken out, and the cambium stem cells were separated and transferred to the subculture medium for culture, and subcultured once every two weeks to obtain Korean ginseng cambium stem cells;
[0051] 2) Collect the culture medium after culturing the Korean ginseng cambium stem cells, filte...
Embodiment 3
[0060] An embodiment of the preparation method of the Korean ginseng stem cell extract sunscreen skin care product of the present invention, the preparation method of the Korean ginseng stem cell extract sunscreen skin care product described in this embodiment is:
[0061] (1) Preparation of Korean ginseng stem cell extract
[0062] 1) Isolation and culture of Korean ginseng cambium stem cells
[0063] After the root tuber of Korean ginseng is sterilized, cut into semicircular slices including the cambium;
[0064] Place the semicircular slices on the WPM medium and culture in the dark at 25°C;
[0065] Two weeks later, the external proliferating bodies with obvious cambium proliferation were taken out, and the cambium stem cells were separated and transferred to the subculture medium for culture, and subcultured once every two weeks to obtain Korean ginseng cambium stem cells;
[0066] 2) Collect the culture medium after culturing the Korean ginseng cambium stem cells, filt...
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