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A kind of fluorescein-rhodamine b copper ion ultraviolet molecular probe for naked eye detection and its synthesis and application method

A molecular probe and synthesis method technology, applied in chemical instruments and methods, fluorescence/phosphorescence, material analysis by optical means, etc., can solve problems such as high price, and achieve the effects of low price, simple detection method and simple steps

Active Publication Date: 2019-05-21
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention will solve existing Cu 2+ The probe needs to be detected by an expensive high-precision fluorescence spectrometer and is easily interfered by the external environment. 2+ Technical problems of molecular probes, and provide a kind of fluorescein-rhodamine B copper ion ultraviolet molecular probes for naked eye detection and its synthesis and use method

Method used

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  • A kind of fluorescein-rhodamine b copper ion ultraviolet molecular probe for naked eye detection and its synthesis and application method
  • A kind of fluorescein-rhodamine b copper ion ultraviolet molecular probe for naked eye detection and its synthesis and application method
  • A kind of fluorescein-rhodamine b copper ion ultraviolet molecular probe for naked eye detection and its synthesis and application method

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specific Embodiment approach 1

[0027] Specific embodiment one: the structural formula of the fluorescein-rhodamine B copper ion ultraviolet molecular probe used for naked eye detection in this embodiment is:

[0028]

specific Embodiment approach 2

[0029] Specific embodiment two: the synthetic method of the fluorescein-rhodamine B copper ion ultraviolet molecular probe that is used for naked-eye detection described in specific embodiment one is as follows:

[0030] 2. According to the molar ratio of fluorescein monoaldehyde and rhodamine B hydrazine as 1: (1 ~ 5), respectively add fluorescein monoaldehyde and rhodamine B hydrazine to alcohol solvent, heat to boiling and reflux, and react for 6 ~ 10h, after the end of the reaction, it was cooled and concentrated to obtain a reaction mixture;

[0031] 2. Take a column chromatography column filled with 200-300 mesh silica gel, use the mixture of dichloromethane and methanol as the eluent, wash the mixture through column chromatography gradient gradient, and separate to obtain Fluorescein-rhodamine B copper ion ultraviolet molecular probe.

specific Embodiment approach 3

[0032] Embodiment 3: The difference between this embodiment and Embodiment 2 is that the alcohol solvent in step 1 is methanol, ethanol, propanol, isopropanol or butanol; the others are the same as Embodiment 2.

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Abstract

The invention discloses a fluorescein-rhodamine B copper ion ultraviolet molecular probe applied to naked eye detection and synthesizing and using methods thereof, relates to a Cu<2+> probe and synthesis and application thereof and aims at solving the technical problems that an existing Cu<2+> molecular probe needs to use an expensive-price high-precision fluorescence spectrophotometer for detection and is prone to being interfered by external environment. A structural formula of the molecular probe disclosed by the invention is shown in a following description. According to a preparation method of the molecular probe, the molecular probe is prepared by the steps of utilizing fluorescein mono-aldehyde and rhodamine B hydrazine to react and utilizing a mixed solvent of methylene dichlorideand methyl alcohol to separate through a column chromatography. A use method of the molecular probe comprises the steps of dissolving the probe in a mixed solution of MeOH and HEPES buffer solution, detecting an added sample to be detected and judging that Cu<2+> is contained if color of the solution is changed into deep pink from light pink; or detecting ultraviolet spectrum absorbance values before and after the sample is added and judging that the sample solution contains the Cu<2+> if the absorbance value is improved when the wavelength is 502 and 553nm. A detection method is simple, quickand visual.

Description

technical field [0001] The present invention relates to Cu 2+ Synthesis and application of molecular probes for detection. Background technique [0002] Cu 2+ An essential trace element widely distributed in biological tissues, Cu 2+ Insufficient or overdose can lead to a series of neurological diseases, such as Menkes syndrome, Alzheimer's disease, Wilson's disease and Parkinson's disease. At the same time Cu 2+ It is also one of the common pollutants in industrial environments. According to the report of the World Health Organization (WHO), Cu 2+ The standard content in reference water should be lower than 20μmol / L. Therefore, it can accurately and quantitatively detect Cu in physiological environment and natural environment. 2+ The content is of great significance, the current detection of Cu 2+ Among the many methods, the spectroscopic analysis method has attracted the attention of scholars because of its high selectivity and high sensitivity. The spectroscopic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D519/00C09K11/06G01N21/64
CPCC07D519/00C09K11/06C09K2211/1029C09K2211/1088G01N21/643G01N2021/6417
Inventor 赵冰卜凡强何玉倩李少鑫尹浩纯阚伟王丽艳
Owner QIQIHAR UNIVERSITY
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