A kind of fluorescein-rhodamine b copper ion ultraviolet molecular probe for naked eye detection and its synthesis and application method
A molecular probe and synthesis method technology, applied in chemical instruments and methods, fluorescence/phosphorescence, material analysis by optical means, etc., can solve problems such as high price, and achieve the effects of low price, simple detection method and simple steps
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
specific Embodiment approach 1
[0027] Specific embodiment one: the structural formula of the fluorescein-rhodamine B copper ion ultraviolet molecular probe used for naked eye detection in this embodiment is:
[0028]
specific Embodiment approach 2
[0029] Specific embodiment two: the synthetic method of the fluorescein-rhodamine B copper ion ultraviolet molecular probe that is used for naked-eye detection described in specific embodiment one is as follows:
[0030] 2. According to the molar ratio of fluorescein monoaldehyde and rhodamine B hydrazine as 1: (1 ~ 5), respectively add fluorescein monoaldehyde and rhodamine B hydrazine to alcohol solvent, heat to boiling and reflux, and react for 6 ~ 10h, after the end of the reaction, it was cooled and concentrated to obtain a reaction mixture;
[0031] 2. Take a column chromatography column filled with 200-300 mesh silica gel, use the mixture of dichloromethane and methanol as the eluent, wash the mixture through column chromatography gradient gradient, and separate to obtain Fluorescein-rhodamine B copper ion ultraviolet molecular probe.
specific Embodiment approach 3
[0032] Embodiment 3: The difference between this embodiment and Embodiment 2 is that the alcohol solvent in step 1 is methanol, ethanol, propanol, isopropanol or butanol; the others are the same as Embodiment 2.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com