Raman spectrum analysis apparatus and method thereof

Abstract

The invention provides a Raman spectrum analysis apparatus and a method thereof. The apparatus comprises a chromatobar, a chromatography cylinder, a clamping device and a Raman spectrometer, wherein the chromatobar is coated with a solid-phase adsorbing material as a chromatographic material; the chromatography cylinder is used for containing a developing solvent; the clamping device is used for fixing the chromatobar at a position to be tested; and the Raman spectrometer is used for scanning chromatogram points of the chromatobar and carrying out Raman spectrum analysis. Laser of the Raman spectrometer is facing towards the chromatobar. The method comprises the following steps: a sample is spotted to a sample spotting area positioned at one end of the chromatobar; the chromatobar is placed into the chromatography cylinder containing the developing solvent such that the sample spotting area is positioned above the liquid level of the developing solvent, and the sample undergoes chromatographic separation; and through the direction of the laser of the Raman spectrometer and relative motion of a mobile device, the chromatobar is moved or rotated after chromatographic separation suchthat the Raman spectrometer conducts scanning of Raman spectrum. The apparatus and the method of the invention are helpful for realizing efficient, fast and interference-free chromatographic separation.

Description

technical field [0001] The invention relates to the field of Raman spectrum analysis, in particular to a Raman spectrum analysis device and method. Background technique [0002] Raman spectroscopy is a technique used to detect the difference between the wavelength of scattered light and the excitation light after a substance is excited by photons of a specific wavelength. Generally, the Raman spectrum of a substance molecule has very similar characteristics to its infrared absorption spectrum, but because the detection process is basically not affected by the shape of the sample and the solvent, it is simpler and more convenient than the infrared spectrum. In recent years, it has been gradually used in pharmaceuticals, Environment, public safety and other fields. [0003] Raman spectroscopy usually detects fingerprints, and its ability to identify a single component from a mixture of substances is very limited. It can be seen that a certain separation of the mixture before...

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Problems solved by technology

Rod thin-layer chromatography combined with flame ionization detection has strong quantitative performance, but the structure of the analyte is easily destroyed during the ionization process, losing structural information

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